Table 1. CD11b − DCs, but not CD11b or... | American Society of Hematology

Table 1.

CD11b⁻DCs, but not CD11b⁺ or plasmacytoid-derived DCs, secrete bioactive IL-12

Bioactive IL-12 p70 secreted (pg/mL) in each of 3 experiments
Experiment	1	2	3
CD11b⁻DCs	542	105	1111
CD11b⁺DCs	84	< 16	< 16
Plasmacytoid cells	< 16	< 16	< 16
Plasmacytoid-derived DCs	< 16	< 16	< 16

Bioactive IL-12 p70 secreted (pg/mL) in each of 3 experiments
Experiment	1	2	3
CD11b⁻DCs	542	105	1111
CD11b⁺DCs	84	< 16	< 16
Plasmacytoid cells	< 16	< 16	< 16
Plasmacytoid-derived DCs	< 16	< 16	< 16

CD11b⁻ dendritic cells (DCs), CD11b⁺DCs, and plasmacytoid-derived DCs were stimulated for 2 days with granulocyte/macrophage–colony stimulating factor (GM-CSF), interferon γ, interleukin (IL)-4, and soluble CD40 ligand (CD40L). Supernatants were then recovered, and IL-12 p70 was measured by enzyme-linked immunoabsorbent assay. IL-12 p70 production by plasmacytoid cells was measured after 2 days of culture with IL-3, GM-CSF, and soluble CD40L.

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