Flow cytometric analysis of K562/cE+pBn, K562/cE+B3, K562/cE+B3SAO, and K562/cE+B3EQ clones
| Clone . | FL1 for mouse negative control . | FL1 for BRIC 71 anti–band 3 . | FL1 for BRIC 69 anti-Rh30 . | FL1 for LA18.18 anti-RhAG . | FL1 for human negative control . | FL1 for MS37 anti-Rhc . | FL1 for H4-1 G-4 anti-RhE . |
|---|---|---|---|---|---|---|---|
| K562/cE+pBn clone 1 | 3.3 | 3.4 | 68 | 48 | 2.4 | 12 | 24 |
| K562/cE+pBn clone 2 | 4.3 | 4.0 | 65 | 47 | 2.8 | 10 | 19 |
| K562/cE+pBn clone 3 | 4.5 | 5.1 | 60 | 45 | 3.2 | 10 | 17 |
| K562/cE+B3 clone 1 | 5.4 | 327 | 228 | 202 | 4.8 | 26 | 45 |
| K562/cE+B3 clone 2 | 4.4 | 54 | 115 | 117 | 4.4 | 21 | 33 |
| K562/cE+B3 clone 3 | 4.2 | 40 | 134 | 145 | 3.7 | 22 | 38 |
| K562/cE+B3 clone 4 | 3.2 | 21 | 161 | 285 | 3.3 | 20 | 32 |
| K562/cE+B3SAO clone 1 | 5.2 | 108 | 352 | 377 | 5.6 | 40 | 39 |
| K562/cE+B3SAO clone 2 | 4.4 | 35 | 189 | 287 | 4.1 | 27 | 32 |
| K562/cE+B3SAO clone 3 | 4.2 | 25 | 184 | 213 | 4.0 | 33 | 39 |
| K562/cE+B3SAO clone 4 | 3.8 | 15 | 198 | 220 | 3.8 | 25 | 28 |
| K562/cE+B3EQ clone 1 | 4.0 | 197 | 232 | 168 | 3.9 | 26 | 31 |
| K562/cE+B3EQ clone 2 | 3.7 | 103 | 159 | 129 | 3.4 | 21 | 30 |
| K562/cE+B3EQ clone 3 | 5.4 | 67 | 134 | 130 | 5.0 | 20 | 28 |
| K562/cE+B3EQ clone 4 | 4.7 | 29 | 205 | 282 | 4.8 | 23 | 38 |
| Clone . | FL1 for mouse negative control . | FL1 for BRIC 71 anti–band 3 . | FL1 for BRIC 69 anti-Rh30 . | FL1 for LA18.18 anti-RhAG . | FL1 for human negative control . | FL1 for MS37 anti-Rhc . | FL1 for H4-1 G-4 anti-RhE . |
|---|---|---|---|---|---|---|---|
| K562/cE+pBn clone 1 | 3.3 | 3.4 | 68 | 48 | 2.4 | 12 | 24 |
| K562/cE+pBn clone 2 | 4.3 | 4.0 | 65 | 47 | 2.8 | 10 | 19 |
| K562/cE+pBn clone 3 | 4.5 | 5.1 | 60 | 45 | 3.2 | 10 | 17 |
| K562/cE+B3 clone 1 | 5.4 | 327 | 228 | 202 | 4.8 | 26 | 45 |
| K562/cE+B3 clone 2 | 4.4 | 54 | 115 | 117 | 4.4 | 21 | 33 |
| K562/cE+B3 clone 3 | 4.2 | 40 | 134 | 145 | 3.7 | 22 | 38 |
| K562/cE+B3 clone 4 | 3.2 | 21 | 161 | 285 | 3.3 | 20 | 32 |
| K562/cE+B3SAO clone 1 | 5.2 | 108 | 352 | 377 | 5.6 | 40 | 39 |
| K562/cE+B3SAO clone 2 | 4.4 | 35 | 189 | 287 | 4.1 | 27 | 32 |
| K562/cE+B3SAO clone 3 | 4.2 | 25 | 184 | 213 | 4.0 | 33 | 39 |
| K562/cE+B3SAO clone 4 | 3.8 | 15 | 198 | 220 | 3.8 | 25 | 28 |
| K562/cE+B3EQ clone 1 | 4.0 | 197 | 232 | 168 | 3.9 | 26 | 31 |
| K562/cE+B3EQ clone 2 | 3.7 | 103 | 159 | 129 | 3.4 | 21 | 30 |
| K562/cE+B3EQ clone 3 | 5.4 | 67 | 134 | 130 | 5.0 | 20 | 28 |
| K562/cE+B3EQ clone 4 | 4.7 | 29 | 205 | 282 | 4.8 | 23 | 38 |
B3SAO indicates band 3 found in Southeast Asian ovalocytosis; clone 1, clone with the highest obtained level of band 3 expression; Rh30, Rh polypeptides; RhAG, Rh glycoprotein.