Quantification of band 3 expression for K562/B3, K562/B3SAO, and K562/B3EQ clones
| Cell parameter . | RBC . | K562/B3 positive control . | K562/B3SAO clone 1 . | K562/B3SAO clone 2 . | K562/B3EQ clone 1 . | K562/B3EQ clone 2 . |
|---|---|---|---|---|---|---|
| Mean cell volume (μm3) | 78 | 2808 | 2843 | 3477 | 2363 | 3489 |
| Mean cell surface area (μm2) | 142 | 963 | 970 | 1110 | 858 | 1113 |
| Band 3 surface fraction | 100% | 40.9% | 35.8% | 40.4% | 35.3% | 32.8% |
| Relative amount of band 3 per PCV | 1(*) | 1/50.8 | 1/17.9 | 1/49.7 | 1/61.6 | 1/38.4 |
| Relative surface density of band 3 | 1(*) | 1/23.4 | 1/9.36 | 1/21.5 | 1/34.8 | 1/20.5 |
| Cell parameter . | RBC . | K562/B3 positive control . | K562/B3SAO clone 1 . | K562/B3SAO clone 2 . | K562/B3EQ clone 1 . | K562/B3EQ clone 2 . |
|---|---|---|---|---|---|---|
| Mean cell volume (μm3) | 78 | 2808 | 2843 | 3477 | 2363 | 3489 |
| Mean cell surface area (μm2) | 142 | 963 | 970 | 1110 | 858 | 1113 |
| Band 3 surface fraction | 100% | 40.9% | 35.8% | 40.4% | 35.3% | 32.8% |
| Relative amount of band 3 per PCV | 1(*) | 1/50.8 | 1/17.9 | 1/49.7 | 1/61.6 | 1/38.4 |
| Relative surface density of band 3 | 1(*) | 1/23.4 | 1/9.36 | 1/21.5 | 1/34.8 | 1/20.5 |
B3SAO indicates band 3 found in Southeast Asian ovalocytosis; RBC, red blood cell; PCV, packed cell volume.
Arbitrarily defined value for RBCs.