The expression of the 7A6 apoptotic mitochondrial antigen is increased by lymphotactin
| Activation (d 2) . | Percent of apo2.7-positive cells . | |
|---|---|---|
| Without Lptn . | With Lptn . | |
| CD3 | 32 (7) | 51 (12.7) |
| CD3 + anti-Lptn | ND | 33.5 (2.2) |
| CD3 + PT | 29 (3) | 29 (8.6) |
| CD3 + IL-2 | 38 (17) | 41 (18) |
| Activation (d 2) . | Percent of apo2.7-positive cells . | |
|---|---|---|
| Without Lptn . | With Lptn . | |
| CD3 | 32 (7) | 51 (12.7) |
| CD3 + anti-Lptn | ND | 33.5 (2.2) |
| CD3 + PT | 29 (3) | 29 (8.6) |
| CD3 + IL-2 | 38 (17) | 41 (18) |
CD4+ T cells were activated for 2 days with CD3 mAb, alone or in presence of the indicated reagents, as described in “Materials and methods.” After washing and permeabilization, staining of the cells with PE-apo2.7 mAb was performed and analyzed by flow cytometry. Bold numbers represent the mean percent values of positive cells from 4 independent experiments, with SD indicated between brackets.
Lptn indicates lymphotactin; ND, not determined; IL-2, interleukin 2.