Detection of donor cells in adult mice after in utero transplantation of transgenic fetal liver cells
| Organs . | Distribution of positive cells . | Transplantation groups3-150 . | |||
|---|---|---|---|---|---|
| AA4.1− GF− 2 mo3-151 . | AA4.1+ GF− 2 mo3-151 . | AA4.1+ GF− > 6 mo3-151 . | AA4.1+ GF+ > 6 mo3-151 . | ||
| Spleen3-152 | Single cells | 6/6 | 22/22 | 3/3 | 15/15 |
| Clusters | 4/6 | 11/22 | 3/3 | 6/15 | |
| Bone marrow3-153 | Single cells | 4/6 | 12/21 | 1/3 | 3/15 |
| Clusters | 0/6 | 3/21 | 0/3 | 0/15 | |
| Liver3-152 | Single cells | 6/6 | 13/20 | 1/3 | 3/15 |
| Brain3-152 | Single cells | 0/6 | 0/22 | 0/3 | 0/15 |
| Organs . | Distribution of positive cells . | Transplantation groups3-150 . | |||
|---|---|---|---|---|---|
| AA4.1− GF− 2 mo3-151 . | AA4.1+ GF− 2 mo3-151 . | AA4.1+ GF− > 6 mo3-151 . | AA4.1+ GF+ > 6 mo3-151 . | ||
| Spleen3-152 | Single cells | 6/6 | 22/22 | 3/3 | 15/15 |
| Clusters | 4/6 | 11/22 | 3/3 | 6/15 | |
| Bone marrow3-153 | Single cells | 4/6 | 12/21 | 1/3 | 3/15 |
| Clusters | 0/6 | 3/21 | 0/3 | 0/15 | |
| Liver3-152 | Single cells | 6/6 | 13/20 | 1/3 | 3/15 |
| Brain3-152 | Single cells | 0/6 | 0/22 | 0/3 | 0/15 |
AA4.1+ indicates FLC column enriched for hematopoietic stem and progenitor cells before transplantation. GF+ indicates FLCs were cultured for 4 days in the presence of growth factors (IL-3, IL-6, and stem cell factor) before transplantation.
Age at analysis.
In the spleens, livers, and brains, multiple sections were assayed from each tissue to obtain a representative sampling of the whole tissue.
In bone marrow, only a small sample from one bone was assayed for each animal, and the assays do not represent the whole bone marrow. Thus, it is likely, given low total numbers of donor cells, that positive areas were missed in the negative mice.