Mobilization of hematopoietic progenitor subsets after treatment with SB-251353, granulocyte colony-stimulating factor, and the combination of SB-251353 and granulocyte colony-stimulating factor
| Progenitor subset . | Mobilization method . | |||
|---|---|---|---|---|
| PBS alone . | SB-251353 alone . | G-CSF alone . | SB-251353 + G-CSF . | |
| CFU-G | 43 ± 14 | 179 ± 46 | 662 ± 291 | 1842 ± 6453-150 |
| CFU-GM | 136 ± 28 | 513 ± 130 | 1807 ± 611 | 3075 ± 479 |
| BFU-E | 18 ± 11 | 102 ± 49 | 188 ± 86 | 368 ± 151 |
| CFU-GEMM | 14 ± 10 | 58 ± 23 | 68 ± 49 | 292 ± 192 |
| CFU-Meg | 35 ± 12 | 147 ± 48 | 378 ± 161 | 1469 ± 6333-150 |
| Progenitor subset . | Mobilization method . | |||
|---|---|---|---|---|
| PBS alone . | SB-251353 alone . | G-CSF alone . | SB-251353 + G-CSF . | |
| CFU-G | 43 ± 14 | 179 ± 46 | 662 ± 291 | 1842 ± 6453-150 |
| CFU-GM | 136 ± 28 | 513 ± 130 | 1807 ± 611 | 3075 ± 479 |
| BFU-E | 18 ± 11 | 102 ± 49 | 188 ± 86 | 368 ± 151 |
| CFU-GEMM | 14 ± 10 | 58 ± 23 | 68 ± 49 | 292 ± 192 |
| CFU-Meg | 35 ± 12 | 147 ± 48 | 378 ± 161 | 1469 ± 6333-150 |
BDF1 mice were injected with either PBS, 2.5 mg/kg SB-251353 SQ, 50 μg/kg G-CSF SQ bid for 4 days, or the combination of G-CSF followed by a single administration of SB-251353 16 hours after the last dose of G-CSF. Blood samples were collected 15 minutes after injection of PBS or SB-251353. Progenitor subtypes were determined as defined in “Materials and methods.” Data are presented as the mean ± SEM per mL blood of 3-4 experiments. P < .05 compared with PBS for all other mobilization methods.
PBS indicates phosphate-buffered saline; G-CSF, granulocyte colony-stimulating factor; CFU, colony-forming unit; G, granulocyte; GM, granulocyte-macrophage; BFU-E, burst forming unit-erythrocyte; GEMM, granulocyte-erythrocyte-monocyte-megakaryocyte; Meg, megakaryocyte.
P < .05 compared with G-CSF alone.