Kinetics of hematopoietic progenitor cell mobilization in mice injected with a single subcutaneous dose of SB-251353
| . | Minutes after SB-251353 administration . | ||||
|---|---|---|---|---|---|
| 0 . | 15 . | 30 . | 45 . | 60 . | |
| CFU-GM/mL blood | 48 ± 4 | 486 ± 38 | 269 ± 44 | 134 ± 16 | 117 ± 15 |
| . | Minutes after SB-251353 administration . | ||||
|---|---|---|---|---|---|
| 0 . | 15 . | 30 . | 45 . | 60 . | |
| CFU-GM/mL blood | 48 ± 4 | 486 ± 38 | 269 ± 44 | 134 ± 16 | 117 ± 15 |
BDF1 mice (n = 3 per time point per experiment) were injected SQ with 2.5 mg/kg SB-251353 and bled at the indicated times. Day 14 CFU-GM (more than 50 cells) stimulated by 10 ng/mL rmIL-1α, 10 ng/mL rmGM-CSF, and 50 ng/mL rmSCF was enumerated in triplicate plates per experiment. Data are presented as the mean ± SEM of 13 experiments.
CFU-GM indicates colony-forming unit granulocyte-macrophage; SQ, subcutaneous; rmGM-CSF, recombinant murine granulocyte-macrophage colony-stimulating factor; SCF, stem cell factor.