Thymocyte proliferation to concanavalin A stimulation with or without cytokines
| Experiment . | . | n . | Medium . | ConA . | ConA + IL-2 . | ConA + IL-4 . | ConA + IL-7 . | PMA + ionomycin . |
|---|---|---|---|---|---|---|---|---|
| 1 | γc+ | 1 | 53 ± 10 | 1052 ± 212 | 13 962 ± 1812 | 10 575 ± 682 | 4088 ± 234 | 27 312 ± 2057 |
| mγc-BMT | 1 | 53 ± 14 | 1349 ± 231 | 2864 ± 74 | 4291 ± 503 | 2309 ± 212 | 35 899 ± 182 | |
| XSCID | 1 | 64 ± 10 | 271 ± 69 | 244 ± 64 | 228 ± 16 | 279 ± 61 | ND | |
| 2 | γc+ | 1 | 67 ± 4 | 1587 ± 147 | 26 434 ± 1249 | 15 363 ± 3493 | 4823 ± 61 | ND |
| mγc-BMT | 1 | 70 ± 7 | 1218 ± 48 | 1756 ± 254 | 1685 ± 86 | 1740 ± 229 | ND | |
| XSCID | 4 | 58 ± 7 | 85 ± 9 | 71 ± 4 | 81 ± 11 | 77 ± 12 | 2788 ± 518 | |
| 3 | γc+ | 1 | 42 ± 7 | 1484 ± 579 | 16 516 ± 94 | 7842 ± 1037 | 5683 ± 739 | 9183 ± 1417 |
| mγc-BMT | 2 | 48 ± 11 | 765 ± 32 | 1181 ± 615 | 1134 ± 550 | 994 ± 600 | 8086 ± 2102 |
| Experiment . | . | n . | Medium . | ConA . | ConA + IL-2 . | ConA + IL-4 . | ConA + IL-7 . | PMA + ionomycin . |
|---|---|---|---|---|---|---|---|---|
| 1 | γc+ | 1 | 53 ± 10 | 1052 ± 212 | 13 962 ± 1812 | 10 575 ± 682 | 4088 ± 234 | 27 312 ± 2057 |
| mγc-BMT | 1 | 53 ± 14 | 1349 ± 231 | 2864 ± 74 | 4291 ± 503 | 2309 ± 212 | 35 899 ± 182 | |
| XSCID | 1 | 64 ± 10 | 271 ± 69 | 244 ± 64 | 228 ± 16 | 279 ± 61 | ND | |
| 2 | γc+ | 1 | 67 ± 4 | 1587 ± 147 | 26 434 ± 1249 | 15 363 ± 3493 | 4823 ± 61 | ND |
| mγc-BMT | 1 | 70 ± 7 | 1218 ± 48 | 1756 ± 254 | 1685 ± 86 | 1740 ± 229 | ND | |
| XSCID | 4 | 58 ± 7 | 85 ± 9 | 71 ± 4 | 81 ± 11 | 77 ± 12 | 2788 ± 518 | |
| 3 | γc+ | 1 | 42 ± 7 | 1484 ± 579 | 16 516 ± 94 | 7842 ± 1037 | 5683 ± 739 | 9183 ± 1417 |
| mγc-BMT | 2 | 48 ± 11 | 765 ± 32 | 1181 ± 615 | 1134 ± 550 | 994 ± 600 | 8086 ± 2102 |
Shown are the mean values ± SEM of incorporated [3H]-thymidine (cpm) obtained from triplicate cultures.
ConA indicates concanavalin A; IL, interleukin; ND, not determined; for other abbreviations, see Table 1.