Mitogen-induced proliferative responses of splenocytes
| Experiment . | . | n . | Medium . | LPS . | CD3 + CD28 . | PMA + ionomycin . |
|---|---|---|---|---|---|---|
| XSCID | 1 | 323 ± 51 | 10 021 ± 1504 | 1203 ± 118 | 3564 ± 446 | |
| 1 | γc+ | 1 | 664 ± 3 | 135 343 ± 6844 | 67 522 ± 6813 | 47 717 ± 3485 |
| mγc-BMT | 1 | 594 ± 53 | 105 925 ± 808 | 28 072 ± 1086 | 27 651 ± 812 | |
| XSCID | 4 | 547 ± 138 | 3560 ± 933 | 1226 ± 179 | 2176 ± 712 | |
| 2 | γc+ | 1 | 225 ± 41 | 120 746 ± 4263 | 5183 ± 1432 | 33 840 ± 1864 |
| mγc-BMT | 2 | 638 ± 117 | 67 360 ± 31 299 | 14 795 ± 262 | 18 617 ± 4974 |
| Experiment . | . | n . | Medium . | LPS . | CD3 + CD28 . | PMA + ionomycin . |
|---|---|---|---|---|---|---|
| XSCID | 1 | 323 ± 51 | 10 021 ± 1504 | 1203 ± 118 | 3564 ± 446 | |
| 1 | γc+ | 1 | 664 ± 3 | 135 343 ± 6844 | 67 522 ± 6813 | 47 717 ± 3485 |
| mγc-BMT | 1 | 594 ± 53 | 105 925 ± 808 | 28 072 ± 1086 | 27 651 ± 812 | |
| XSCID | 4 | 547 ± 138 | 3560 ± 933 | 1226 ± 179 | 2176 ± 712 | |
| 2 | γc+ | 1 | 225 ± 41 | 120 746 ± 4263 | 5183 ± 1432 | 33 840 ± 1864 |
| mγc-BMT | 2 | 638 ± 117 | 67 360 ± 31 299 | 14 795 ± 262 | 18 617 ± 4974 |
Shown are the mean values ± SEM of incorporated [3H]-thymidine (cpm) obtained from triplicate cultures.
LPS indicates lipopolysaccharide; PMA, phorbol myristate acetate; XSCID, untreated γc+ X-linked severe combined immunodeficiency mice; γc+, untreated normal mice; mγc-BMT, Δγc+-XSCID mice transplanted with mγc-transduced bone marrow cells.