Expansion of clones from fetal-liver cells infected with the J2 retrovirus, according to genotype
| Culture . | Genotype . | |
|---|---|---|
| EKLF−/− . | EKLF+/− . | |
| 96-well | 35/96 | 14/96 |
| 24-well | 25/96 | 5/96 |
| 6-well | 17/96 | 1/96* |
| Frozen cell lines† | 15/96‡ | 1/96* |
| Culture . | Genotype . | |
|---|---|---|
| EKLF−/− . | EKLF+/− . | |
| 96-well | 35/96 | 14/96 |
| 24-well | 25/96 | 5/96 |
| 6-well | 17/96 | 1/96* |
| Frozen cell lines† | 15/96‡ | 1/96* |
Values are the numbers of clones that expanded in a total of 96.
This single clone from EKLF+/− fetal liver was maintained in continuous culture for 6 weeks, during which it was always composed of a mixture of adherent macrophages and nonadherent cells.
Cells were frozen after they reached a density of 5 × 105 cells/mL in 5 mL medium.
All these clones from EKLF−/− fetal liver had erythroid morphologic features.