Erythroblast-conditioned medium stimulation of monocyte migration
| Lower compartment . | Upper compartment . | ||
|---|---|---|---|
| None . | ECMa . | ECMf . | |
| None | 7300 ± 1000 | 7000 ± 1000 | 7500 ± 1000 |
| ECMa | 26 000 ± 2000 | 7800 ± 1000 | 8000 ± 1000 |
| ECMf | 190 000 ± 8000 | 105 000 ± 5000 | 6800 ± 1000 |
| Lower compartment . | Upper compartment . | ||
|---|---|---|---|
| None . | ECMa . | ECMf . | |
| None | 7300 ± 1000 | 7000 ± 1000 | 7500 ± 1000 |
| ECMa | 26 000 ± 2000 | 7800 ± 1000 | 8000 ± 1000 |
| ECMf | 190 000 ± 8000 | 105 000 ± 5000 | 6800 ± 1000 |
Two 105 monocytes were placed in the upper compartment of a modified Boyden chamber. ECM was added in the lower compartment (lower compartment, rows ECMa and ECMf) or not added (lower compartment, first row). ECM was added in the upper compartment (upper compartment, columns ECMa and ECMf) or not added (upper compartment, column none). ECMa contained 50 pg/mL P1GF and 1.2 μg/mL VEGF-A, and ECMf contained 750 pg/mL P1GF and 18 μg/mL VEGF-A. Results are mean numbers (±SD) of migrated monocytes. Each experiment has been performed 2 times.
ECM indicates erythroblast-conditioned medium.