Quantitative analysis of human repopulating cells after 4 and 6 days of ex vivo culture in the presence or absence of soluble hDelta-1 or hDelta-4
| . | Day 4 . | Day 6 . | ||||
|---|---|---|---|---|---|---|
| No. positive . | No. negative . | No. positive out of total transplanted (% engrafted) . | No. positive . | No. negative . | No. positive out of total transplanted (% engrafted) . | |
| Control-treated | 4 | 13 | 4/17 (23%) | 4 | 5 | 4/9 (44%) |
| Delta-1 | 9 | 8 | 9/17* (53%) | 4 | 6 | 4/10 (40%) |
| Control-treated | 2 | 10 | 2/12 (17%) | 1 | 5 | 1/6 (17%) |
| Delta-4 | 2 | 10 | 2/12 (17%) | 0 | 5 | 0/5 (0%) |
| . | Day 4 . | Day 6 . | ||||
|---|---|---|---|---|---|---|
| No. positive . | No. negative . | No. positive out of total transplanted (% engrafted) . | No. positive . | No. negative . | No. positive out of total transplanted (% engrafted) . | |
| Control-treated | 4 | 13 | 4/17 (23%) | 4 | 5 | 4/9 (44%) |
| Delta-1 | 9 | 8 | 9/17* (53%) | 4 | 6 | 4/10 (40%) |
| Control-treated | 2 | 10 | 2/12 (17%) | 1 | 5 | 1/6 (17%) |
| Delta-4 | 2 | 10 | 2/12 (17%) | 0 | 5 | 0/5 (0%) |
Single wells containing 500 to 2500 highly purified CD34+CD38−Lin− human hematopoietic cells were isolated by flow cytometric sorting and cultured for 4 and 6 days in the presence of optimized combinations of hematopoietic growth factors previously shown to sustain repopulating cells. Mice were transplanted with cells harvested at the indicated time points, and the number of mice containing human hematopoietic cells (positive) was compared.
Significant difference, P < .01.