PCR analysis of MLV DNA in PBMCs from animals after transplantation with autologous CD34+ cells transduced with LZRS/GFP
| Mm 96-97 . | Mm 254-97 . | Mm 278-97 . | Mm 280-97 . | Mm 250-97 . | Mm 256-97 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Week . | MLV . | Week . | MLV . | Week . | MLV . | Week . | MLV . | Week . | MLV . | Week . | MLV . |
| 0 | <0.01 | 0 | <0.01 | 0 | <0.01 | 0 | <0.01 | 0 | <0.01 | 0 | <0.01 |
| 1 | 0.10 | 2 | 0.02 | 1 | 0.01 | 1 | 0.03 | 1 | 0.03 | 1 | 0.12 |
| 3 | 0.15 | 3 | 0.02 | 2 | 0.02 | 2 | <0.01 | 2 | <0.01 | 3 | 0.06 |
| 11 | 0.04 | 11 | <0.01 | 8 | <0.01 | 7 | <0.01 | 7 | <0.01 | 12 | <0.01 |
| 18 | 0.02 | 17 | <0.01 | 15 | <0.01 | 21 | <0.01 | 18 | <0.01 | 18 | <0.01 |
| 40 | <0.01 | 21 | <0.01 | 25 | <0.01 | 35 | <0.01 | 35 | <0.01 | 27 | <0.01 |
| 48 | <0.01 | — | — | — | — | — | — | — | — | — | — |
| Mm 96-97 . | Mm 254-97 . | Mm 278-97 . | Mm 280-97 . | Mm 250-97 . | Mm 256-97 . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Week . | MLV . | Week . | MLV . | Week . | MLV . | Week . | MLV . | Week . | MLV . | Week . | MLV . |
| 0 | <0.01 | 0 | <0.01 | 0 | <0.01 | 0 | <0.01 | 0 | <0.01 | 0 | <0.01 |
| 1 | 0.10 | 2 | 0.02 | 1 | 0.01 | 1 | 0.03 | 1 | 0.03 | 1 | 0.12 |
| 3 | 0.15 | 3 | 0.02 | 2 | 0.02 | 2 | <0.01 | 2 | <0.01 | 3 | 0.06 |
| 11 | 0.04 | 11 | <0.01 | 8 | <0.01 | 7 | <0.01 | 7 | <0.01 | 12 | <0.01 |
| 18 | 0.02 | 17 | <0.01 | 15 | <0.01 | 21 | <0.01 | 18 | <0.01 | 18 | <0.01 |
| 40 | <0.01 | 21 | <0.01 | 25 | <0.01 | 35 | <0.01 | 35 | <0.01 | 27 | <0.01 |
| 48 | <0.01 | — | — | — | — | — | — | — | — | — | — |
MLV DNA was assessed by a real-time PCR assay using primers and probes specific for the MLV ψ packaging sequence (modified from Gerard et al21). Values are expressed as the percentage of transduced cells. The lower limit of detection of this assay is 0.01% MLV-transduced cells.