Table 1. Cell yield and transduction... | American Society of Hematology

Table 1.

Cell yield and transduction efficiency of rhesus macaque CD34⁺ bone marrow cells obtained with or without cytokine treatment

Animal	Priming	CD34⁺cells harvested (×10⁶)	CD34⁺cells transplanted (×10⁶)	Transplanted CD34⁺ cells/kg (×10⁶)	% Transduction
Animal	Priming	CD34⁺cells harvested (×10⁶)	CD34⁺cells transplanted (×10⁶)	Transplanted CD34⁺ cells/kg (×10⁶)	Flow	CFU
Mm 96-97	None	9.2	24	10	14	23
Mm 254-97	None	4.4	9	3.9	41	53
Mm 278-97	G-CSF	10.8	25	8.1	13	9
Mm 280-97	G-CSF	8.8	16	5.2	31	10
Mm 250-97	G-CSF, SCF	7.0	45	14.1	58	66
Mm 256-97	G-CSF, SCF	8.0	36	13.3	57	58

Animal	Priming	CD34⁺cells harvested (×10⁶)	CD34⁺cells transplanted (×10⁶)	Transplanted CD34⁺ cells/kg (×10⁶)	% Transduction
Animal	Priming	CD34⁺cells harvested (×10⁶)	CD34⁺cells transplanted (×10⁶)	Transplanted CD34⁺ cells/kg (×10⁶)	Flow	CFU
Mm 96-97	None	9.2	24	10	14	23
Mm 254-97	None	4.4	9	3.9	41	53
Mm 278-97	G-CSF	10.8	25	8.1	13	9
Mm 280-97	G-CSF	8.8	16	5.2	31	10
Mm 250-97	G-CSF, SCF	7.0	45	14.1	58	66
Mm 256-97	G-CSF, SCF	8.0	36	13.3	57	58

Percent transduction of bone marrow cells was assessed by flow cytometry 24 hours after the third round of transduction with LZRS/eGFP. Percent transduction of CFU was assessed using an inverted fluorescent microscope after 7 to 10 days in methylcellulose culture.

G-CSF indicates granulocyte colony-stimulating factor; SCF, stem cell factor.

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