Patient clinical characteristics
| Patient . | Age (y)/sex . | Other autoimmune disorders . | Splenectomy . | Antiplatelet autoantibody* . | |
|---|---|---|---|---|---|
| Anti-αIIbβ3 . | Anti-GPIb/IX . | ||||
| ITP-1 | 61/M | No | 892.6 | Neg | |
| ITP-2 | 37/F | Yes | 81.3 | Neg | |
| ITP-3 | 24/F | Yes | 104.1 | Neg | |
| ITP-4 | 46/M | APL | Yes | 228.0 | Neg |
| ITP-5 | 33/F | AIHA, APL | Yes | 286.9 | Neg |
| ITP-6 | 23/F | CD | Yes | 76.9 | Neg |
| ITP-7 | 47/F | Yes | 46.9 | Neg | |
| ITP-8 | 44/F | AIHA | Yes | 135.8 | Neg |
| ITP-9 | 70/F | AIHA | No | 130.4 | 44.0 |
| ITP-10 | 44/F | Yes | 1210.8 | Neg | |
| ITP-11 | 23/M | Yes | 238.8 | Neg | |
| ITP-12 | 53/M | Yes | 149.2 | Neg | |
| ITP-13 | 24/M | Yes | 59.1 | Neg | |
| ITP-14 | 20/F | Yes | 7.7 | Neg | |
| ITP-15 | 32/M | Yes | Neg | 23.94 | |
| Drug-Ab | 85/M | No | Neg | 685.6 | |
| PTP-1 | 73/F | No | — | — | |
| Patient . | Age (y)/sex . | Other autoimmune disorders . | Splenectomy . | Antiplatelet autoantibody* . | |
|---|---|---|---|---|---|
| Anti-αIIbβ3 . | Anti-GPIb/IX . | ||||
| ITP-1 | 61/M | No | 892.6 | Neg | |
| ITP-2 | 37/F | Yes | 81.3 | Neg | |
| ITP-3 | 24/F | Yes | 104.1 | Neg | |
| ITP-4 | 46/M | APL | Yes | 228.0 | Neg |
| ITP-5 | 33/F | AIHA, APL | Yes | 286.9 | Neg |
| ITP-6 | 23/F | CD | Yes | 76.9 | Neg |
| ITP-7 | 47/F | Yes | 46.9 | Neg | |
| ITP-8 | 44/F | AIHA | Yes | 135.8 | Neg |
| ITP-9 | 70/F | AIHA | No | 130.4 | 44.0 |
| ITP-10 | 44/F | Yes | 1210.8 | Neg | |
| ITP-11 | 23/M | Yes | 238.8 | Neg | |
| ITP-12 | 53/M | Yes | 149.2 | Neg | |
| ITP-13 | 24/M | Yes | 59.1 | Neg | |
| ITP-14 | 20/F | Yes | 7.7 | Neg | |
| ITP-15 | 32/M | Yes | Neg | 23.94 | |
| Drug-Ab | 85/M | No | Neg | 685.6 | |
| PTP-1 | 73/F | No | — | — | |
Polystyrene beads coated with mAb against either αIIbβ3 or GPIb/IX and blocked with bovine serum albumin were incubated with solubilized platelet lysate from either patients or control subjects. Bound immune complexes were detected with radiolabeled anti-IgG. Results are expressed as ratio:cpm anti-IgG bound to patient sample/mean cpm bound to 3 control samples (normal <2.0).7
ITP, immune thrombocytopenic purpura; Drug-Ab, drug-dependent (procainamide) antibody; PTP, posttransfusion purpura; APL, antiphospholipid syndrome; AIHA, autoimmune hemolytic anemia; CD, Crohn disease of the colon.