Response of thymic emigrants in secondary MLR
| C57Bl/6 responder T cells (1 × 105) . | Primary stimulation3-150 . | Secondary stimulation dendritic cells (×104) . | Incorporation of 3H-TdR (SD) . |
|---|---|---|---|
| Thymic emigrants | C57Bl/6 | — | 388 (136) |
| C57Bl/6 (0.2) | 1239 (309) | ||
| (1.0) | 1308 (201) | ||
| (5.0) | 6911 (311) | ||
| Balb/c (0.2) | 9988 (497) | ||
| (1.0) | 14 234 (724) | ||
| (5.0) | 27 856 (2009) | ||
| Balb/c | — | 285 (136) | |
| C57Bl/6 (0.2) | 1001 (136) | ||
| (1.0) | 6846 (397) | ||
| (5.0) | 11 203 (1028) | ||
| Balb/c (0.2) | 32 285 (2941) | ||
| (1.0) | 62 831 (3985) | ||
| (5.0) | 88 027 (4784) | ||
| Splenic T cells | C57Bl/6 | — | 361 (111) |
| C57Bl/6 (0.2) | 881 (204) | ||
| (1.0) | 1458 (109) | ||
| (5.0) | 11 117 (899) | ||
| Balb/c (0.2) | 12 811 (3213) | ||
| (1.0) | 12 004 (4112) | ||
| (5.0) | 28 294 (1297) | ||
| Balb/c | — | 272 (39) | |
| C57Bl/6 (0.2) | 1822 (140) | ||
| (1.0) | 4311 (348) | ||
| (5.0) | 5776 (1126) | ||
| Balb/c (0.2) | 32 801 (4414) | ||
| (1.0) | 69 318 (2233) | ||
| (5.0) | 96 682 (4709) |
| C57Bl/6 responder T cells (1 × 105) . | Primary stimulation3-150 . | Secondary stimulation dendritic cells (×104) . | Incorporation of 3H-TdR (SD) . |
|---|---|---|---|
| Thymic emigrants | C57Bl/6 | — | 388 (136) |
| C57Bl/6 (0.2) | 1239 (309) | ||
| (1.0) | 1308 (201) | ||
| (5.0) | 6911 (311) | ||
| Balb/c (0.2) | 9988 (497) | ||
| (1.0) | 14 234 (724) | ||
| (5.0) | 27 856 (2009) | ||
| Balb/c | — | 285 (136) | |
| C57Bl/6 (0.2) | 1001 (136) | ||
| (1.0) | 6846 (397) | ||
| (5.0) | 11 203 (1028) | ||
| Balb/c (0.2) | 32 285 (2941) | ||
| (1.0) | 62 831 (3985) | ||
| (5.0) | 88 027 (4784) | ||
| Splenic T cells | C57Bl/6 | — | 361 (111) |
| C57Bl/6 (0.2) | 881 (204) | ||
| (1.0) | 1458 (109) | ||
| (5.0) | 11 117 (899) | ||
| Balb/c (0.2) | 12 811 (3213) | ||
| (1.0) | 12 004 (4112) | ||
| (5.0) | 28 294 (1297) | ||
| Balb/c | — | 272 (39) | |
| C57Bl/6 (0.2) | 1822 (140) | ||
| (1.0) | 4311 (348) | ||
| (5.0) | 5776 (1126) | ||
| Balb/c (0.2) | 32 801 (4414) | ||
| (1.0) | 69 318 (2233) | ||
| (5.0) | 96 682 (4709) |
For primary stimulation, responder T cell (3 × 106/mL) were mixed with irradiated dendritic cells (2 × 106/mL) and cultured for 2 days. Viable cells were separated by Ficoll-Hypaque centrifugation, rested for 1 day, then tested in a secondary MLR.