Concentration of colony-forming units–culture in bone marrow Lin−/kit++/LFA-1+ and Lin−/kit++/LFA-1− cells
| . | Bone marrow (Lin−) . | |
|---|---|---|
| Lin−/kit++/LFA-1+(17.3%)* . | Lin−/kit++/LFA-1−(6.3%) . | |
| CFU-C, % | 24.7 ± 1.03 | 45.7 ± 5.45 |
| BFU-E, % | 3.2 | 0 |
| Total CFU-C fraction in Lin− cells† | 0.6 | 0.4 |
| . | Bone marrow (Lin−) . | |
|---|---|---|
| Lin−/kit++/LFA-1+(17.3%)* . | Lin−/kit++/LFA-1−(6.3%) . | |
| CFU-C, % | 24.7 ± 1.03 | 45.7 ± 5.45 |
| BFU-E, % | 3.2 | 0 |
| Total CFU-C fraction in Lin− cells† | 0.6 | 0.4 |
CFU-C indicates colony-forming units–culture; BFU-E, burst-forming units–erythroid; FACS, fluorescence-activated cell sorter; MC, methylcellulose.
Bone marrow mononuclear cells were depleted of lineage+cells to derive Lin− populations. These were subsequently labeled with directly conjugated anti-kit and anti–LFA-1 antibodies. Lin−/kit++/LFA-1+ cells (17.3% of Lin− cells) and Lin−/kit++/LFA-1− cells (6.3%) were FACS-sorted and cultured in MC media.
CFU-C fraction was estimated from percentage of CFU-C in each fraction and percentage of total cells in that fraction.