Colony formation from various sources
| Cell source . | No. of colonies/5 × 104 cells . | ||||||
|---|---|---|---|---|---|---|---|
| E . | Mk . | EMk . | Mk-Mix . | Others . | Total . | ||
| Pr . | Ad . | ||||||
| 7.5 dpc YS | 27 ± 3 | 7 ± 2 | 0 | 2 ± 1 | 5 ± 2 | 7 ± 2 | 48 ± 4 |
| 7.5 dpc EB | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 8.5 dpc YS | 56 ± 5 | 62 ± 6 | 0 | 13 ± 3 | 24 ± 5 | 39 ± 5 | 194 ± 15 |
| 8.5 dpc EB | 21 ± 3 | 4 ± 2 | 0 | 2 ± 1 | 5 ± 3 | 6 ± 2 | 38 ± 5 |
| 10.5 dpc YS | 78 ± 15 | 16 ± 4 | 10 ± 3 | 18 ± 4 | 32 ± 5 | 125 ± 17 | 279 ± 25 |
| 10.5 dpc EB | 8 ± 3 | 1 ± 1 | 1 ± 0 | 1 ± 1 | 3 ± 1 | 11 ± 2 | 25 ± 5 |
| 13.5 dpc FL | 652 ± 64 | 1 ± 1 | 84 ± 9 | 18 ± 6 | 26 ± 6 | 478 ± 35 | 1258 ± 115 |
| Adult BM | 31 ± 4 | 0 | 5 ± 2 | 2 ± 1 | 22 ± 3 | 146 ± 12 | 206 ± 7 |
| Cell source . | No. of colonies/5 × 104 cells . | ||||||
|---|---|---|---|---|---|---|---|
| E . | Mk . | EMk . | Mk-Mix . | Others . | Total . | ||
| Pr . | Ad . | ||||||
| 7.5 dpc YS | 27 ± 3 | 7 ± 2 | 0 | 2 ± 1 | 5 ± 2 | 7 ± 2 | 48 ± 4 |
| 7.5 dpc EB | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 8.5 dpc YS | 56 ± 5 | 62 ± 6 | 0 | 13 ± 3 | 24 ± 5 | 39 ± 5 | 194 ± 15 |
| 8.5 dpc EB | 21 ± 3 | 4 ± 2 | 0 | 2 ± 1 | 5 ± 3 | 6 ± 2 | 38 ± 5 |
| 10.5 dpc YS | 78 ± 15 | 16 ± 4 | 10 ± 3 | 18 ± 4 | 32 ± 5 | 125 ± 17 | 279 ± 25 |
| 10.5 dpc EB | 8 ± 3 | 1 ± 1 | 1 ± 0 | 1 ± 1 | 3 ± 1 | 11 ± 2 | 25 ± 5 |
| 13.5 dpc FL | 652 ± 64 | 1 ± 1 | 84 ± 9 | 18 ± 6 | 26 ± 6 | 478 ± 35 | 1258 ± 115 |
| Adult BM | 31 ± 4 | 0 | 5 ± 2 | 2 ± 1 | 22 ± 3 | 146 ± 12 | 206 ± 7 |
5 × 104 cells from various sources were cultured in the presence of 6 factors (SCF, IL-3, IL-6, G-CSF, EPO and TPO). Mk colonies were classified into primitive-type (Pr) and adult-type (Ad) as shown in the text. The number of colonies indicates mean ± SD in triplicate cultures.