Immunophenotyping of PTLD by flow cytometry
| Animal no. . | % CD3-CD16-lymphocytes . | Immunophenotype . | ||||||
|---|---|---|---|---|---|---|---|---|
| Origin . | CD2 . | CD3 . | CD16 . | Class II . | IgM . | Igκ . | ||
| 12 757 | 50 (PBMC) | Host | Dim | — | ND | ND | ND | ND |
| 13 100 | 57 (PBMC) | Host | Dim | — | — | ND | ND | ND |
| 13 318 | > 70 (LN) | Host | Dim | — | — | + | + | + |
| 13 271 | > 95 (LN) | Host | Dim | — | — | + | + | — |
| 13 432 | > 70 (LN) | Host | Dim | — | — | + | + | — |
| 13 433 | 47 (LN) | Host | Dim | — | — | + | + | + |
| 13 629 | 40 (LN) | Donor | Dim | — | — | + | Dim | + |
| 13 813 | > 95 (LN) | Host | Dim | — | — | + | + | + |
| 13 801 | 85 (LN) | Host | Dim | — | — | + | + | + |
| Animal no. . | % CD3-CD16-lymphocytes . | Immunophenotype . | ||||||
|---|---|---|---|---|---|---|---|---|
| Origin . | CD2 . | CD3 . | CD16 . | Class II . | IgM . | Igκ . | ||
| 12 757 | 50 (PBMC) | Host | Dim | — | ND | ND | ND | ND |
| 13 100 | 57 (PBMC) | Host | Dim | — | — | ND | ND | ND |
| 13 318 | > 70 (LN) | Host | Dim | — | — | + | + | + |
| 13 271 | > 95 (LN) | Host | Dim | — | — | + | + | — |
| 13 432 | > 70 (LN) | Host | Dim | — | — | + | + | — |
| 13 433 | 47 (LN) | Host | Dim | — | — | + | + | + |
| 13 629 | 40 (LN) | Donor | Dim | — | — | + | Dim | + |
| 13 813 | > 95 (LN) | Host | Dim | — | — | + | + | + |
| 13 801 | 85 (LN) | Host | Dim | — | — | + | + | + |
Analysis was done on PBMC or lymph node (LN) on day of autopsy or maximal lymphoproliferation as indicated in Table 1.
Dim indicates diminished; ND, not determined.
Surface negative—confirmed positive by cytoplasmic staining.