Sequence analysis of the rearranged IgH gene in KSHV-positive cells
| Case no. . | Lesion . | Total no.of clones . | Clones with identical VH . | Clones with identical CDR3 . | Mutation in VH . |
|---|---|---|---|---|---|
| 1 | Lymphoma (LN) | 8 | 8 (DP75) | 5 | 1s |
| Microlymphoma 1 (Sp) | 8 | 5 (DP14) | 4 | 1s | |
| 1 (DP75) | Identical to the dominant lymphoma clone | 1s | |||
| 4 (DP10) | 2 | 1R | |||
| Microlymphoma 2 (Sp) | 9 | 3 (DP14) | 1s | ||
| 1 (DP75) | Identical to the dominant lymphoma clone | 1s | |||
| 2 (V3-64) | 2 | — | |||
| 2 (DP77) | 2 | 1R | |||
| 1 (DP49) | — | ||||
| Microlymphoma 1 (Sp) | 8 | 1 (DP47) | 2R | ||
| 1 (V3-53) | 1R | ||||
| 1 (Cos-8) | — | ||||
| 3 (DP47) | 2 | — | |||
| 2 (DP77) | — | ||||
| 2 | Microlymphoma 2 (Sp) | 10 | 2 (DP38) | — | |
| 1 (DP54) | — | ||||
| 1 (DP53) | 2s | ||||
| 3 (DP42) | — | ||||
| 2 (8−1B+) | 1s | ||||
| Microlymphoma 3 (Sp) | 8 | 1 (DP58) | — | ||
| 1 (DP63) | — | ||||
| 1 (Cos-8) | 1R, 1s | ||||
| 2 (DP71) | 2 | 3s | |||
| 3 (DP77) | 2R | ||||
| 1 (DP49) | 1s | ||||
| 3 | Microlymphoma 1 (LN) | 10 | 2 (DP47) | — | |
| 1 (V3-64) | 1R | ||||
| 1 (DP54) | 1R | ||||
| 3 (DP49) | — | ||||
| 2 (DP47) | — | ||||
| Microlymphoma 2 (LN) | 7 | 1 (DP63) | 1R | ||
| 1 (4.30) | 1R | ||||
| 4 (DP78) | 4 | — | |||
| 4 | Micro-lymphoma 1 (Sp) | 10 | 3 (DP46) | 3 | 1R |
| 1 (DP47) | — |
| Case no. . | Lesion . | Total no.of clones . | Clones with identical VH . | Clones with identical CDR3 . | Mutation in VH . |
|---|---|---|---|---|---|
| 1 | Lymphoma (LN) | 8 | 8 (DP75) | 5 | 1s |
| Microlymphoma 1 (Sp) | 8 | 5 (DP14) | 4 | 1s | |
| 1 (DP75) | Identical to the dominant lymphoma clone | 1s | |||
| 4 (DP10) | 2 | 1R | |||
| Microlymphoma 2 (Sp) | 9 | 3 (DP14) | 1s | ||
| 1 (DP75) | Identical to the dominant lymphoma clone | 1s | |||
| 2 (V3-64) | 2 | — | |||
| 2 (DP77) | 2 | 1R | |||
| 1 (DP49) | — | ||||
| Microlymphoma 1 (Sp) | 8 | 1 (DP47) | 2R | ||
| 1 (V3-53) | 1R | ||||
| 1 (Cos-8) | — | ||||
| 3 (DP47) | 2 | — | |||
| 2 (DP77) | — | ||||
| 2 | Microlymphoma 2 (Sp) | 10 | 2 (DP38) | — | |
| 1 (DP54) | — | ||||
| 1 (DP53) | 2s | ||||
| 3 (DP42) | — | ||||
| 2 (8−1B+) | 1s | ||||
| Microlymphoma 3 (Sp) | 8 | 1 (DP58) | — | ||
| 1 (DP63) | — | ||||
| 1 (Cos-8) | 1R, 1s | ||||
| 2 (DP71) | 2 | 3s | |||
| 3 (DP77) | 2R | ||||
| 1 (DP49) | 1s | ||||
| 3 | Microlymphoma 1 (LN) | 10 | 2 (DP47) | — | |
| 1 (V3-64) | 1R | ||||
| 1 (DP54) | 1R | ||||
| 3 (DP49) | — | ||||
| 2 (DP47) | — | ||||
| Microlymphoma 2 (LN) | 7 | 1 (DP63) | 1R | ||
| 1 (4.30) | 1R | ||||
| 4 (DP78) | 4 | — | |||
| 4 | Micro-lymphoma 1 (Sp) | 10 | 3 (DP46) | 3 | 1R |
| 1 (DP47) | — |
Clones with frequent mutations (> 10) were occasionally seen. They are most likely from reactive B cells rather than KSHV-positive plasmablasts and are not included.
S indicates silent mutation; R, replacement change; —, no mutation; LN, lymph node; Sp, spleen.