Primers used for Ig λ light-chain PCR
| Target region . | Primer* . | |
|---|---|---|
| Name . | Sequence . | |
| VL1 | 5′ CAGTCTGTGCTGAC(T/G)CAGCC 3′ | |
| VL2 | 5′ CAGTCTGCCCTGACTCAGCC 3′ | |
| VL3 | 5′ TCCTATGAGCTGAC(A/T)CAG 3′ | |
| Fr1 | VL4ab | 5′ CAGCCTGTGCTGACTCAATC 3′ |
| VL5-9-10 | 5′ CAG(C/G)CTGTGCTGACTCAGCC 3′ | |
| VL6 | 5′ AATTTTATGCTGACTCAGCC 3′ | |
| VL7-8 | 5′ CAG(A/G)CTGTGGTGACTCAGGA 3′ | |
| Fr3 | FR3 | 5′ GA(T/C)GAGGCTGA(T/C)TATTACTG 3′ |
| Jλ | JL1 | 5′ AGGACGGTGACCTTGGTCCC 3′ |
| JL2-3-7 | 5′ AGGACGGTCAGCT(G/T)GGT(C/G)CC 3′ | |
| Target region . | Primer* . | |
|---|---|---|
| Name . | Sequence . | |
| VL1 | 5′ CAGTCTGTGCTGAC(T/G)CAGCC 3′ | |
| VL2 | 5′ CAGTCTGCCCTGACTCAGCC 3′ | |
| VL3 | 5′ TCCTATGAGCTGAC(A/T)CAG 3′ | |
| Fr1 | VL4ab | 5′ CAGCCTGTGCTGACTCAATC 3′ |
| VL5-9-10 | 5′ CAG(C/G)CTGTGCTGACTCAGCC 3′ | |
| VL6 | 5′ AATTTTATGCTGACTCAGCC 3′ | |
| VL7-8 | 5′ CAG(A/G)CTGTGGTGACTCAGGA 3′ | |
| Fr3 | FR3 | 5′ GA(T/C)GAGGCTGA(T/C)TATTACTG 3′ |
| Jλ | JL1 | 5′ AGGACGGTGACCTTGGTCCC 3′ |
| JL2-3-7 | 5′ AGGACGGTCAGCT(G/T)GGT(C/G)CC 3′ | |
Primers were designed according to the consensus sequences of family members of individual light-chain genes.