Oligonucleotides and annealing temperatures used for genotyping polymorphisms located in the promoter and the 3′UTR of the TAFI gene by allele-specific PCR
| Polymorphism . | Primer sequences direct/reverse (D/R) . | PCR size (bp) . | Primer allele-specific Sequences . | PCR Allele-specific size (bp) . | Annealing temperature (°C) . |
|---|---|---|---|---|---|
| C-2599G | D:CCTCATAAAGCAGTGCAAC | 296 | C:CTAAAGGAATCATATCCA | 235 | 55 |
| R:GTATGCTGTATTCTGGGTG | G:CTAAAGGAATCATATCGA | ||||
| − 2345 2G/1G | D:CACCTCAACTGGACTATGT | 404 | 1G:AGTTTTTAAAACATGAAAGA | 293 | 54 |
| R:TGTTCCCTTGCAGTTTAGC | 2G:AGTTTTTAAAACATGAAAGGA | ||||
| A-1690G | D:CACCTGTAGACTTTTGC | 357 | A:TTAACTATTTTGACTGTTTA | 95 | 55 |
| R:CACTGAAGGAGAGAAAG | G:TTAACTATTTTGACTGTTTG | ||||
| G-1102T | D:CAGTGGAGGTCACTTTA | 311 | G:AGTTTGAGACCATCCAGG | 151 | 57 |
| R:ACTGCAACCTTCACTCT | T:AGTTTGAGACCATCCAGT | ||||
| G-438A | D:ACCAATGGGAATGTAGG | 698 | A:GAGGAACTCACTTTTTACAT | 301 | 60 |
| R:TTGGCTTAGTTAGGCTG | G:GAGGAACTCACTTTTTACGT | ||||
| Ala147Thr | A:GTTTCTGGAAAAGAACAAA | 105 | 58 | ||
| R:ATGGCCTATGAACCACAAGC | G:GTTTCTGGAAAAGAACAAG | 59 | |||
| C+1542G | D:CCAGCAAGACCAAATCA | 408 | C:AGTCAAACGTCGAAACT | 238 | 55 |
| R:ATTACCGTGGAGCAAAC | G:AGTCAAACGTCGAAAGT | ||||
| T+1583A | D:CTTGGCATGTCATTAGG | 612 | A:CTCAAGCAAGTTTAATCAAA | 294 | 57 |
| R:TGCGGCATTTGTTGACA | T:CTCAAGCAAGTTTAATCAAT | 55 |
| Polymorphism . | Primer sequences direct/reverse (D/R) . | PCR size (bp) . | Primer allele-specific Sequences . | PCR Allele-specific size (bp) . | Annealing temperature (°C) . |
|---|---|---|---|---|---|
| C-2599G | D:CCTCATAAAGCAGTGCAAC | 296 | C:CTAAAGGAATCATATCCA | 235 | 55 |
| R:GTATGCTGTATTCTGGGTG | G:CTAAAGGAATCATATCGA | ||||
| − 2345 2G/1G | D:CACCTCAACTGGACTATGT | 404 | 1G:AGTTTTTAAAACATGAAAGA | 293 | 54 |
| R:TGTTCCCTTGCAGTTTAGC | 2G:AGTTTTTAAAACATGAAAGGA | ||||
| A-1690G | D:CACCTGTAGACTTTTGC | 357 | A:TTAACTATTTTGACTGTTTA | 95 | 55 |
| R:CACTGAAGGAGAGAAAG | G:TTAACTATTTTGACTGTTTG | ||||
| G-1102T | D:CAGTGGAGGTCACTTTA | 311 | G:AGTTTGAGACCATCCAGG | 151 | 57 |
| R:ACTGCAACCTTCACTCT | T:AGTTTGAGACCATCCAGT | ||||
| G-438A | D:ACCAATGGGAATGTAGG | 698 | A:GAGGAACTCACTTTTTACAT | 301 | 60 |
| R:TTGGCTTAGTTAGGCTG | G:GAGGAACTCACTTTTTACGT | ||||
| Ala147Thr | A:GTTTCTGGAAAAGAACAAA | 105 | 58 | ||
| R:ATGGCCTATGAACCACAAGC | G:GTTTCTGGAAAAGAACAAG | 59 | |||
| C+1542G | D:CCAGCAAGACCAAATCA | 408 | C:AGTCAAACGTCGAAACT | 238 | 55 |
| R:ATTACCGTGGAGCAAAC | G:AGTCAAACGTCGAAAGT | ||||
| T+1583A | D:CTTGGCATGTCATTAGG | 612 | A:CTCAAGCAAGTTTAATCAAA | 294 | 57 |
| R:TGCGGCATTTGTTGACA | T:CTCAAGCAAGTTTAATCAAT | 55 |