Time-dependent release of NO from GSNO
| . | Release NO (nM) . | |
|---|---|---|
| GSNO (100 μM) . | GSNO (1 mM) . | |
| 1 h | 420 ± 20 | 2520 ± 950 |
| 2 h | 170 ± 30 | 2270 ± 1030 |
| 4 h | 20 ± 20 | 1620 ± 560 |
| . | Release NO (nM) . | |
|---|---|---|
| GSNO (100 μM) . | GSNO (1 mM) . | |
| 1 h | 420 ± 20 | 2520 ± 950 |
| 2 h | 170 ± 30 | 2270 ± 1030 |
| 4 h | 20 ± 20 | 1620 ± 560 |
LLC-PK1 cells were exposed to 100 μM or 1 mM GSNO, and NO release was continuously measured by an NO electrode. NO values at 1, 2, and 4 hours after addition of GSNO are given. Results (mean values ± SD) are representative for at least 3 independent experiments.