Induction of apoptosis in MDM2-overexpressing stable transfectants
| . | % of apoptotic cells . | ||
|---|---|---|---|
| γ Radiation (8 Gy, 72 h) . | Okadaı̈c acid (20 nM, 48 h) . | Doxorubicin (100 ng/mL, 48 h) . | |
| Saos-2 | 28 ± 5 | 27 ± 7 | 33 ± 4 |
| pCMV D7 | 34 ± 3 | 24 ± 3 | 28 ± 5 |
| pCMV A6 | 21 ± 4 | 21 ± 4 | 13 ± 3 |
| pCMV C7 | 22 ± 3 | 31 ± 7 | 28 ± 4 |
| pCMV G9 | 22 ± 2 | 34 ± 1 | 20 ± 3 |
| mdm2 E7 | 21 ± 3 | 28 ± 5 | 19 ± 4 |
| mdm2 D4 | 29 ± 1 | 28 ± 5 | 35 ± 3 |
| mdm2 F1 | 26 ± 3 | 26 ± 4 | 24 ± 5 |
| mdm2 G1 | 22 ± 5 | 23 ± 5 | 20 ± 5 |
| mdm2 B7 | 15 ± 3 | 24 ± 5 | 22 ± 6 |
| . | % of apoptotic cells . | ||
|---|---|---|---|
| γ Radiation (8 Gy, 72 h) . | Okadaı̈c acid (20 nM, 48 h) . | Doxorubicin (100 ng/mL, 48 h) . | |
| Saos-2 | 28 ± 5 | 27 ± 7 | 33 ± 4 |
| pCMV D7 | 34 ± 3 | 24 ± 3 | 28 ± 5 |
| pCMV A6 | 21 ± 4 | 21 ± 4 | 13 ± 3 |
| pCMV C7 | 22 ± 3 | 31 ± 7 | 28 ± 4 |
| pCMV G9 | 22 ± 2 | 34 ± 1 | 20 ± 3 |
| mdm2 E7 | 21 ± 3 | 28 ± 5 | 19 ± 4 |
| mdm2 D4 | 29 ± 1 | 28 ± 5 | 35 ± 3 |
| mdm2 F1 | 26 ± 3 | 26 ± 4 | 24 ± 5 |
| mdm2 G1 | 22 ± 5 | 23 ± 5 | 20 ± 5 |
| mdm2 B7 | 15 ± 3 | 24 ± 5 | 22 ± 6 |
The percentage of apoptotic cells was obtained by FACS analysis of cells labeled with annexin V-FITC and PI and after subtraction of the percentage of dead cells in negative controls (samples cultured in the same conditions but without the apoptotic inducers) from the percentages of dead cells obtained after treatments. The data shown represent the average values (±SD) from 3 independent experiments.