Effects of mdm2 AS on cell viability
| . | % of apoptotic cells . | ||
|---|---|---|---|
| Medium . | mdm2 SC . | mdm2 AS . | |
| BL2 | 20 ± 3 | 25 ± 8 | 54 ± 9 |
| Priess | 28 ± 1 | 33 ± 3 | 44 ± 2 |
| Ramos | 28 ± 6 | 30 ± 2 | 31 ± 3 |
| Saos-2 | 25 ± 4 | 25 ± 3 | 22 ± 5 |
| . | % of apoptotic cells . | ||
|---|---|---|---|
| Medium . | mdm2 SC . | mdm2 AS . | |
| BL2 | 20 ± 3 | 25 ± 8 | 54 ± 9 |
| Priess | 28 ± 1 | 33 ± 3 | 44 ± 2 |
| Ramos | 28 ± 6 | 30 ± 2 | 31 ± 3 |
| Saos-2 | 25 ± 4 | 25 ± 3 | 22 ± 5 |
Twenty-four hours after electropermeabilization without oligodeoxynucleotide (medium), with a scrambled (SC) mdm2 oligodeoxynucleotide, or with an antisense (AS) mdm2 oligodeoxynucleotide, the percentage of apoptotic cells was obtained by FACS analysis of cells labeled with annexin V-FITC and PI. The data shown represent the average values (±SD) from 3 independent experiments.