Results indicating failure of early growth response gene 1–infected, myeloid-enriched bone marrow cells to prevent death in lethally irradiated mice
| Cells transplanted* . | Mice surviving (%)† . | No. of spleen colonies‡ . | No. of femoral BM cells (×106)1-153 . | Results of colony assays according to cell type1-154 . | ||||
|---|---|---|---|---|---|---|---|---|
| No. of colonies1-155 . | Blast (%) . | Granulocytes (%) . | Macrophages (%) . | Others (%) . | ||||
| BM normal | 100 | 14 | 4.0 | 60 | 30 | 43 | 25 | 2 |
| BM mock | 90 | 10 | 2.9 | 65 | 60 | 25 | 11 | 3 |
| BMneo | 90 | 12 | 2.2 | 50 | 65 | 22 | 12 | 1 |
| BMEgr-1 | 0 | 0 | 0.001 | — | — | — | — | |
| Cells transplanted* . | Mice surviving (%)† . | No. of spleen colonies‡ . | No. of femoral BM cells (×106)1-153 . | Results of colony assays according to cell type1-154 . | ||||
|---|---|---|---|---|---|---|---|---|
| No. of colonies1-155 . | Blast (%) . | Granulocytes (%) . | Macrophages (%) . | Others (%) . | ||||
| BM normal | 100 | 14 | 4.0 | 60 | 30 | 43 | 25 | 2 |
| BM mock | 90 | 10 | 2.9 | 65 | 60 | 25 | 11 | 3 |
| BMneo | 90 | 12 | 2.2 | 50 | 65 | 22 | 12 | 1 |
| BMEgr-1 | 0 | 0 | 0.001 | — | — | — | — | |
BM indicates bone marrow; Egr-1, early growth response gene 1.
Each BM type was transplanted into 13 lethally irradiated mice (0.2 × 106 cells/mouse).
For each BM type, 10 mice were monitored for survival for 4 weeks. All mice injected with BMEgr-1 died within 10 days after injection.
Spleen colonies were counted on day 8. Values are mean (with SDs up to ±20%) results for spleens obtained from 3 mice.
BM cells were collected from femurs 7 days after transplantation. Values are mean numbers of BM cells (with SDs up to ±15%) obtained from femurs of 3 mice.
Seven days after transplantation, BM cells were recovered from femurs and 0.05 × 106 cells (except in the BMEgr-1 group, in which 0.001 × 106 cells were used) were plated in 1 mL methylcellulose supplemented with interleukin 3 (10% WEHI-3B–conditioned medium). Colony number was determined after 8 days. Values are mean (SD up to ±15%) results in triplicate plates.
Colonies generated after 8 days in methylcellulose were pooled and used to prepare cytospin smears. At least 300 May-Grünwald–stained cells were scored. Percentage values are mean (SD up to ±15%; ie, 30% ± 4.5%) results from 3 smears prepared from triplicate methylcellulose cultures.