Binding kinetics and affinities of isolated single-chain variable domain antibody fragments
| scFv . | kon(M−1s−1) . | koff(s−1) . | Kd (nM) . |
|---|---|---|---|
| KM33 | 1.8 × 106 | 1.9 × 10−4 | 0.1 |
| KM35 | 1.6 × 105 | 9.4 × 10−3 | 59 |
| KM36 | 1.7 × 105 | 9.5 × 10−3 | 55 |
| KM37 | 2.6 × 105 | 9.5 × 10−4 | 3.7 |
| KM38 | 3.8 × 105 | 3.5 × 10−3 | 9.0 |
| KM41 | 1.8 × 105 | 2.9 × 10−4 | 1.6 |
| scFv . | kon(M−1s−1) . | koff(s−1) . | Kd (nM) . |
|---|---|---|---|
| KM33 | 1.8 × 106 | 1.9 × 10−4 | 0.1 |
| KM35 | 1.6 × 105 | 9.4 × 10−3 | 59 |
| KM36 | 1.7 × 105 | 9.5 × 10−3 | 55 |
| KM37 | 2.6 × 105 | 9.5 × 10−4 | 3.7 |
| KM38 | 3.8 × 105 | 3.5 × 10−3 | 9.0 |
| KM41 | 1.8 × 105 | 2.9 × 10−4 | 1.6 |
Kd, kon, andkoff were determined by surface plasmon resonance as described in “Materials and methods.”