Deduced protein sequences of isolated factor VIII A3-C1–specific single-chain variable domain antibody fragments
| Heavy chains . | |||||||
|---|---|---|---|---|---|---|---|
| . | FR1 . | CDR1 . | FR2 . | CDR2 . | FR3 . | CDR3 . | FR4 . |
| 1 | 1 1 | ||||||
| 1 2 3 | 4 | 5 6 | 7 8 9 | 0 | 0 1 | ||
| 123456789012345678901234567890 | 12345 | 67890123456789 | 012a3456789012345 | 67890123456789012abc345678901234 | 567890abcdefghijklm12 | 34567890123 | |
| DP-14 | QVQLVQSGAEVKKPGASVKVSCKASGYTFT | SYGIS | WVRQAPGQGLEWMG | WISAYNGNTNYAQKLQG | RVTMTTDTSTSTAYMELRSLRSDDTAVYYCAR | ||
| KM37 | ------------------E---------A | T---N | -------------- | -F-PHK-D------F-- | ---L------N--------N------------ | GKADQYYNLWRGSHSPNLLDN | WGQGTTVTVSS |
| DP-15 | QVQLVQSGAEVKKPGASVKVSCKASGYTFT | SYDIN | WVRQATGQGLEWMG | WMNPNSGNTGYAQKFQG | RVTMTRNTSISTAYMELSSLRSEDTAVYYCAR | ||
| KM41 | ----1--a-D------------T----I-- | ----D | -------------- | --------A-F----K- | -L-L--D--T-------RR-E----------- | STPHSYSGSGLPPTSDS.... | WGQGTLVTVSS |
| DP-49 | QVQLVESGGGVVQPGRSLRLSCAASGFTFS | SYGMH | WVRQAPGKGLEWVA | VISYDGSNKYYADSVKG | RFTISRDNSKNTLYLQMNSLRAEDTAVYYCAK | ||
| KM33 | e---------------------VD--L--- | ----- | -------A------ | ------ND--------- | --A-----A-----------TI---------- | DLIESNIAEAF.......... | WGQGTLVTVSS |
| KM38 | --n-r----------K-------------- | D-AI- | ------------L- | -------S--------- | -------------F-------V----L----- | DRHINISPPDS.......... | WGQGTLVTVSS |
| DP-77 | EVQLVESGGGLVKPGGSLRLSCAASGFTFS | SYSMN | WVRQAPGKGLEWVS | SISSSSSYIYYADSVKG | RFTISRDNAKNSLYLQMNSLRAEDTAVYYCAR | ||
| KM35 | ---------D--E---------T------R | R-DIH | ----T--------- | ----GGN--D------- | ---------N-VV-----------M---F--- | DGTIFGSAATWRAFDI..... | WGRGTMVTVSS |
| KM36 | -----------------------------R | R-DIH | ----T--------- | ----GGN--D------- | ---------N-VV-----------M---F--- | DGTIFGSAATWRAFDI..... | WGRGTMVTVSS |
| Heavy chains . | |||||||
|---|---|---|---|---|---|---|---|
| . | FR1 . | CDR1 . | FR2 . | CDR2 . | FR3 . | CDR3 . | FR4 . |
| 1 | 1 1 | ||||||
| 1 2 3 | 4 | 5 6 | 7 8 9 | 0 | 0 1 | ||
| 123456789012345678901234567890 | 12345 | 67890123456789 | 012a3456789012345 | 67890123456789012abc345678901234 | 567890abcdefghijklm12 | 34567890123 | |
| DP-14 | QVQLVQSGAEVKKPGASVKVSCKASGYTFT | SYGIS | WVRQAPGQGLEWMG | WISAYNGNTNYAQKLQG | RVTMTTDTSTSTAYMELRSLRSDDTAVYYCAR | ||
| KM37 | ------------------E---------A | T---N | -------------- | -F-PHK-D------F-- | ---L------N--------N------------ | GKADQYYNLWRGSHSPNLLDN | WGQGTTVTVSS |
| DP-15 | QVQLVQSGAEVKKPGASVKVSCKASGYTFT | SYDIN | WVRQATGQGLEWMG | WMNPNSGNTGYAQKFQG | RVTMTRNTSISTAYMELSSLRSEDTAVYYCAR | ||
| KM41 | ----1--a-D------------T----I-- | ----D | -------------- | --------A-F----K- | -L-L--D--T-------RR-E----------- | STPHSYSGSGLPPTSDS.... | WGQGTLVTVSS |
| DP-49 | QVQLVESGGGVVQPGRSLRLSCAASGFTFS | SYGMH | WVRQAPGKGLEWVA | VISYDGSNKYYADSVKG | RFTISRDNSKNTLYLQMNSLRAEDTAVYYCAK | ||
| KM33 | e---------------------VD--L--- | ----- | -------A------ | ------ND--------- | --A-----A-----------TI---------- | DLIESNIAEAF.......... | WGQGTLVTVSS |
| KM38 | --n-r----------K-------------- | D-AI- | ------------L- | -------S--------- | -------------F-------V----L----- | DRHINISPPDS.......... | WGQGTLVTVSS |
| DP-77 | EVQLVESGGGLVKPGGSLRLSCAASGFTFS | SYSMN | WVRQAPGKGLEWVS | SISSSSSYIYYADSVKG | RFTISRDNAKNSLYLQMNSLRAEDTAVYYCAR | ||
| KM35 | ---------D--E---------T------R | R-DIH | ----T--------- | ----GGN--D------- | ---------N-VV-----------M---F--- | DGTIFGSAATWRAFDI..... | WGRGTMVTVSS |
| KM36 | -----------------------------R | R-DIH | ----T--------- | ----GGN--D------- | ---------N-VV-----------M---F--- | DGTIFGSAATWRAFDI..... | WGRGTMVTVSS |
Sequence numbering is according to Kabat et al.31Sequences are available from GenBank under accession numbers: AF234247(VHKM33); AF234248 (VHKM35); AF234249 (VHKM36); AF234250 (VHKM37);AF234251 (VHKM38); AF234252 (VHKM41); AF234253 (VLKM33); AF234254(VLKM35); AF234255 (VLKM36); AF234256 (VLKM37); AF234257 (VLKM38);AF234258 (VLKM41).
scFv indicates single-chain variable domain antibody fragments; FR, framework region; CDR, complementarity-determining region. Dashes indicate sequence identity to germline. Lowercase indicates amino acid substitutions encoded by the PCR primer.