Most homologous germline gene segments used in A3-C1 specific single-chain variable domain antibody fragments
| Clone . | VHdomain . | VL domain . | |||
|---|---|---|---|---|---|
| Germline . | Family . | Substitutions nucl/aa . | Germline . | Family . | |
| KM37 | DP-14 (1-18) | VH1 | 22/13 | DPK8 (L8) | VκI |
| KM41 | DP-15 (1-8) | VH1 | 21/14 | DPK22 (A27) | VκIII |
| KM33 | DP-49 (3-30) | VH3 | 21/10 | DPK3 (L11) | VκI |
| KM38 | DP-49 (3-30) | VH3 | 23/9 | L12a (L12) | VκI |
| KM35 | DP-77 (3-21) | VH3 | 31/18 | DPL16 (V2-13) | Vλ3 |
| KM36 | DP-77 (3-21) | VH3 | 26/15 | DPL16 (V2-13) | Vλ3 |
| Clone . | VHdomain . | VL domain . | |||
|---|---|---|---|---|---|
| Germline . | Family . | Substitutions nucl/aa . | Germline . | Family . | |
| KM37 | DP-14 (1-18) | VH1 | 22/13 | DPK8 (L8) | VκI |
| KM41 | DP-15 (1-8) | VH1 | 21/14 | DPK22 (A27) | VκIII |
| KM33 | DP-49 (3-30) | VH3 | 21/10 | DPK3 (L11) | VκI |
| KM38 | DP-49 (3-30) | VH3 | 23/9 | L12a (L12) | VκI |
| KM35 | DP-77 (3-21) | VH3 | 31/18 | DPL16 (V2-13) | Vλ3 |
| KM36 | DP-77 (3-21) | VH3 | 26/15 | DPL16 (V2-13) | Vλ3 |
VH and VL germline gene use and nomenclature according to V-BASE.28 The number of different nucleotides and amino acids compared to the nonmutated germlines are indicated.
Nucl indicates nucleotides; aa, amino acids.