Table 4. Chronic B-cell leukemia with... | American Society of Hematology

Table 4.

Chronic B-cell leukemia with potentially functional biallelic Vκ-Jκ rearrangements as determined by Southern blotting

Patient	Vκ	Junctional region^4-150			Jκ	Frame^4-151	RT-PCR^‡	Somatic mutations^4-153
3	Vκ1-37	−3	CT	−6	Jκ4	−	+	−
	NI							NI
11	Vκ1-5	−	−	−	Jκ4	+	+	−
	NA^4-155							NA
12	Vκ2-30	−	−	−3	Jκ1	+	+	−
	Vκ1-39	−3	GG	−3	Jκ2	−	−	−
13	Vκ1-5	−3	−	−3	Jκ1	+	+	−
	NI							NI
14	Vκ1-5	−3	CGC	−1	Jκ2	+	+	−
	Vκ2-28	−	GA	−4	Jκ1	−	+	−
15	Vκ3-20	−1	G	−1	Jκ2	+	+	+
	Vκ3-11	−	T	−	Jκ4	−	−	−
16	Vκ1-39	0	A	−1	Jκ1	+	ND	+
	Vκ1-5	−13	−	−	Jκ4	−	ND	−
17	Vκ3-20	−	−	−	Jκ5	+	+	−
	Vκ1-37	−	TT	−	Jκ3	−	−	−
18	Vκ3-20	−1	GA	−4	Jκ1	+	+	−
	Vκ4-1	−	C	−5	Jκ4	−	+	−
19	Vκ1-27	−3	−	−	Jκ1	+	+	−
	NI							NI

Patient	Vκ	Junctional region^4-150			Jκ	Frame^4-151	RT-PCR^‡	Somatic mutations^4-153
3	Vκ1-37	−3	CT	−6	Jκ4	−	+	−
	NI							NI
11	Vκ1-5	−	−	−	Jκ4	+	+	−
	NA^4-155							NA
12	Vκ2-30	−	−	−3	Jκ1	+	+	−
	Vκ1-39	−3	GG	−3	Jκ2	−	−	−
13	Vκ1-5	−3	−	−3	Jκ1	+	+	−
	NI							NI
14	Vκ1-5	−3	CGC	−1	Jκ2	+	+	−
	Vκ2-28	−	GA	−4	Jκ1	−	+	−
15	Vκ3-20	−1	G	−1	Jκ2	+	+	+
	Vκ3-11	−	T	−	Jκ4	−	−	−
16	Vκ1-39	0	A	−1	Jκ1	+	ND	+
	Vκ1-5	−13	−	−	Jκ4	−	ND	−
17	Vκ3-20	−	−	−	Jκ5	+	+	−
	Vκ1-37	−	TT	−	Jκ3	−	−	−
18	Vκ3-20	−1	GA	−4	Jκ1	+	+	−
	Vκ4-1	−	C	−5	Jκ4	−	+	−
19	Vκ1-27	−3	−	−	Jκ1	+	+	−
	NI							NI

RT-PCR indicates reverse transcriptase–polymerase chain reaction.

F4-150

The junctional region is described as the number of bases lost at the 3′ end of the segment followed by randomly inserted nucleotides and the number of bases lost from the 5′ end of the J segment.

F4-151

Frame of the junctional region: +, in-frame, without stop codons in the junctional region; −, out-of-frame.

‡

ND indicates not determined; +, present; −, absent.

F4-153

+ indicates V segments with more than 2% somatic mutations; −, less than 2% somatic mutations; NI, not identified, because the rearrangement could not be amplified.

F4-155

NA indicates not applicable, because only one intact chromosome 2 was present, although 2 rearrangements seemed to be present in Southern blot analysis (see text).

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