Blocking experiments with anti-CD95-Fas-F(ab)2fragments in primary B-lineage ALL cells
| Patient . | B-lineage ALL (% apoptosis) . | ||||
|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | |
| Medium control | 35.1 ± 5.8 | 54.8 ± 5.3 | 23.9 ± 3.1 | 44.2 ± 3.7 | 38.5 ± 4.2 |
| Epirubicin | 99.1 ± 9.9 | 98.1 ± 9.8 | 92.2 ± 7.2 | 97.5 ± 4.1 | 62.3 ± 6.9 |
| Epirubicin + control F(ab)2 | 99.1 ± 2.9 | 99.2 ± 6.2 | 95.6 ± 6.9 | 98.7 ± 4.7 | 68.4 ± 7.4 |
| Epirubicin + anti-CD95 F(ab)2 | 99.3 ± 5.7 | 95.4 ± 7.9 | 98.6 ± 8.1 | 94.7 ± 3 | 65.4 ± 4.1 |
| Patient . | B-lineage ALL (% apoptosis) . | ||||
|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | |
| Medium control | 35.1 ± 5.8 | 54.8 ± 5.3 | 23.9 ± 3.1 | 44.2 ± 3.7 | 38.5 ± 4.2 |
| Epirubicin | 99.1 ± 9.9 | 98.1 ± 9.8 | 92.2 ± 7.2 | 97.5 ± 4.1 | 62.3 ± 6.9 |
| Epirubicin + control F(ab)2 | 99.1 ± 2.9 | 99.2 ± 6.2 | 95.6 ± 6.9 | 98.7 ± 4.7 | 68.4 ± 7.4 |
| Epirubicin + anti-CD95 F(ab)2 | 99.3 ± 5.7 | 95.4 ± 7.9 | 98.6 ± 8.1 | 94.7 ± 3 | 65.4 ± 4.1 |
B-lineage ALL cells were cultured for 48 hours in the presence or absence of epirubicin (0.1 μg/mL). F(ab)2 fragments were added at 1 μg/mL. Apoptosis was measured on the single-cell level by assessing the nuclear DNA content. Each experiment was performed in triplicate. Mean values for the percentage of apoptotic cells ± SD (n = 3) are shown for 5 patients with childhood B-lineage ALL.