Phenotypic analysis and cytolytic effector function of the nonadherent cell fraction after 2- to 3-week coculture of peripheral blood lymphocytes with autologous dendritic cells pulsed with cytomegalovirus antigen
| Target cell/donor no. . | Days of culture . | % CD8 . | E:T ratios . | Autologous I/P (% lysis) . | Autologous NI/NP (% lysis) . | Allogeneic I/P (% lysis) . | Allogeneic NI/NP (% lysis) . |
|---|---|---|---|---|---|---|---|
| CMV-infected FBs | |||||||
| 1 | 14 | ND | 10:1 | 6.5 | 1.1 | 0.4 | 0 |
| 2 | 13 | 14 | 10:1 | 36.2 | 0 | 4.1 | 0 |
| 20:1 | 39.1 | 0 | 5.1 | 0 | |||
| 3 | 15 | ND | 5:1 | 20.5 | 0 | 0 | 0 |
| 10:1 | 19.9 | 0 | 0 | 0 | |||
| 4 | 13 | 19 | 5:1 | 9.5 | 0 | 4.1 | 0 |
| 10:1 | 12.0 | 0 | 1.6 | 0 | |||
| 20:1 | 15.6 | 0 | 2.9 | 0 | |||
| 5 | 24 | 9 | 5:1 | 1.0 | 2.6 | 4.1 | 0 |
| 10:1 | 6.3 | 0 | 2.3 | 0 | |||
| 20:1 | 6.1 | 0 | 0.1 | 0 | |||
| 6 | 14 | 7 | 10:1 | 1.3 | 0 | 2.8 | 0 |
| 20:1 | 11.1 | 0 | 0 | 0 | |||
| 7 | 13 | ND | 10:1 | 0 | 0.5 | 1.5 | 0 |
| 20:1 | 6.7 | 0.7 | 1.1 | 1.5 | |||
| 8 | 14 | ND | 10:1 | 5.8 | 0 | 3.9 | 1.5 |
| 20:1 | 3.8 | 1.9 | 1.5 | 2.4 | |||
| CMV-infected DCs | |||||||
| 9 | 21 | 17 | 10:1 | 6.0 | 0 | 0 | 0.7 |
| 20:1 | 4.0 | 0 | 5.0 | 2.0 | |||
| 10 | 14 | 25 | 10:1 | 12.5 | 0 | 0 | 3.5 |
| 20:1 | 23.5 | 3.8 | 0.4 | 9.1 | |||
| CMV-antigen-pulsed DCs | |||||||
| 11 | 14 | 47 | 10:1 | 26.0 | 0 | 0 | 0 |
| 20:1 | 35.0 | 2.4 | 2.6 | 0 | |||
| 12 | 14 | 5 | 10:1 | 28.0 | 0 | 1.7 | 0 |
| 20:1 | 42.0 | 0 | 1.5 | 0 | |||
| Unknown* | |||||||
| 13 | 20 | 67 | — | — | — | — | — |
| 14 | 21 | 28 | — | — | — | — | — |
| 15 | 14 | 26 | — | — | — | — | — |
| 18 | 35 | — | — | — | — | — | |
| 16 | 14 | 12 | — | — | — | — | — |
| 17 | 13 | 39 | — | — | — | — | — |
| Target cell/donor no. . | Days of culture . | % CD8 . | E:T ratios . | Autologous I/P (% lysis) . | Autologous NI/NP (% lysis) . | Allogeneic I/P (% lysis) . | Allogeneic NI/NP (% lysis) . |
|---|---|---|---|---|---|---|---|
| CMV-infected FBs | |||||||
| 1 | 14 | ND | 10:1 | 6.5 | 1.1 | 0.4 | 0 |
| 2 | 13 | 14 | 10:1 | 36.2 | 0 | 4.1 | 0 |
| 20:1 | 39.1 | 0 | 5.1 | 0 | |||
| 3 | 15 | ND | 5:1 | 20.5 | 0 | 0 | 0 |
| 10:1 | 19.9 | 0 | 0 | 0 | |||
| 4 | 13 | 19 | 5:1 | 9.5 | 0 | 4.1 | 0 |
| 10:1 | 12.0 | 0 | 1.6 | 0 | |||
| 20:1 | 15.6 | 0 | 2.9 | 0 | |||
| 5 | 24 | 9 | 5:1 | 1.0 | 2.6 | 4.1 | 0 |
| 10:1 | 6.3 | 0 | 2.3 | 0 | |||
| 20:1 | 6.1 | 0 | 0.1 | 0 | |||
| 6 | 14 | 7 | 10:1 | 1.3 | 0 | 2.8 | 0 |
| 20:1 | 11.1 | 0 | 0 | 0 | |||
| 7 | 13 | ND | 10:1 | 0 | 0.5 | 1.5 | 0 |
| 20:1 | 6.7 | 0.7 | 1.1 | 1.5 | |||
| 8 | 14 | ND | 10:1 | 5.8 | 0 | 3.9 | 1.5 |
| 20:1 | 3.8 | 1.9 | 1.5 | 2.4 | |||
| CMV-infected DCs | |||||||
| 9 | 21 | 17 | 10:1 | 6.0 | 0 | 0 | 0.7 |
| 20:1 | 4.0 | 0 | 5.0 | 2.0 | |||
| 10 | 14 | 25 | 10:1 | 12.5 | 0 | 0 | 3.5 |
| 20:1 | 23.5 | 3.8 | 0.4 | 9.1 | |||
| CMV-antigen-pulsed DCs | |||||||
| 11 | 14 | 47 | 10:1 | 26.0 | 0 | 0 | 0 |
| 20:1 | 35.0 | 2.4 | 2.6 | 0 | |||
| 12 | 14 | 5 | 10:1 | 28.0 | 0 | 1.7 | 0 |
| 20:1 | 42.0 | 0 | 1.5 | 0 | |||
| Unknown* | |||||||
| 13 | 20 | 67 | — | — | — | — | — |
| 14 | 21 | 28 | — | — | — | — | — |
| 15 | 14 | 26 | — | — | — | — | — |
| 18 | 35 | — | — | — | — | — | |
| 16 | 14 | 12 | — | — | — | — | — |
| 17 | 13 | 39 | — | — | — | — | — |
The phenotypic analysis was restricted to the percentage of CD8+ cells at the time points indicated. Cytolytic effector function is shown as the percentage of target cells lysed, as determined by lactate dehydrogenase-release cytotoxicity assays done at the same time points.
E:T indicates effector to target; I/P, infected or pulsed with CMV antigen; NI/NP, not infected or not pulsed with CMV antigen; CMV cytomegalovirus; FBs, fibroblasts; DCs, dendritic cells; and ND, not done.
Cytotoxicity assay results were not available for 5 donors.