FL expression in T lymphocytes in response to CD3 and CD28 receptor ligation and PMA treatment
| Treatment . | mFL (MFI ± SEM) . | sFL (pg/mL ± SEM) . |
|---|---|---|
| Ex vivo | 1.4 ± 0.04 | NA |
| No stimulation | 2.5 ± 0.1* | 24.2 ± 2.2† |
| IL-7 | 7.7 ± 0.4* | 79.8 ± 7.1† |
| Anti-CD3/CD28 Abs | 1.6 ± 0.1* | 62.8 ± 2.5† |
| Anti-CD3/CD28 Abs/IL-7 | 2.2 ± 0.1 | 74.1 ± 4.5 |
| PMA | 1.3 ± 0.1* | 60.3 ± 1.2† |
| PMA/IL-7 | 1.6 ± 0.1 | 146.2 ± 11.0 |
| Treatment . | mFL (MFI ± SEM) . | sFL (pg/mL ± SEM) . |
|---|---|---|
| Ex vivo | 1.4 ± 0.04 | NA |
| No stimulation | 2.5 ± 0.1* | 24.2 ± 2.2† |
| IL-7 | 7.7 ± 0.4* | 79.8 ± 7.1† |
| Anti-CD3/CD28 Abs | 1.6 ± 0.1* | 62.8 ± 2.5† |
| Anti-CD3/CD28 Abs/IL-7 | 2.2 ± 0.1 | 74.1 ± 4.5 |
| PMA | 1.3 ± 0.1* | 60.3 ± 1.2† |
| PMA/IL-7 | 1.6 ± 0.1 | 146.2 ± 11.0 |
Levels of mFL and sFL were measured by FACS analysis and ELISA, respectively. For experimental details, see the legend to Figure 4. NA indicates not applicable. Values are expressed as mean (±SEM) of at least 3 separate experiments performed in duplicate.
P < .005 or
P < .01, mFL and sFL expression with respect to the values observed without stimulation (No stimulation).