Comparison analysis of genes expressed in IL-4-primed CB-derived MCs and IL-4-primed PB-derived MCs by using GeneChip
| Accession no. . | Gene name . | MCs . | 4-150Average difference . | Ratio to β-actin . |
|---|---|---|---|---|
| X67325 | CDK inhibitor, p27kip1 | CB-derived | −1442 | −0.0218 |
| X67325 | PB-derived | 9683 | 0.1186 | |
| X06948 | FcεRI α chain | CB-derived | 761 | 0.0115 |
| X06948 | PB-derived | 8215 | 0.1006 | |
| M34996 | HLA-DQA | CB-derived | 2511 | 0.0379 |
| M34996 | PB-derived | 13 949 | 0.1709 | |
| M89796 | FcεRI β chain | CB-derived | 4057 | 0.0613 |
| M89796 | PB-derived | 15 706 | 0.1924 | |
| M63379 | TRPM-2 | CB-derived | 34 450 | 0.5202 |
| M63379 | PB-derived | 123 684 | 1.5150 | |
| X03068 | HLA-DQw1.1 β chain | CB-derived | 2387 | 0.0360 |
| X03068 | PB-derived | 8243 | 0.1010 | |
| HG4069 | Monocyte chemotactic protein | CB-derived | 7113 | 0.1074 |
| HG4069 | PB-derived | 22 056 | 0.2702 | |
| U50136 | Leukotriene C4 synthase | CB-derived | 9581 | 0.1447 |
| U50136 | PB-derived | 26 175 | 0.3206 | |
| J04990 | Cathepsin G | CB-derived | 27 165 | 0.4102 |
| J04990 | PB-derived | 26 756 | 0.3277 | |
| M33493 | Tryptase | CB-derived | 107 446 | 1.6223 |
| M33493 | PB-derived | 123 746 | 1.5158 | |
| M64269 | Chymase | CB-derived | 5556 | 0.0839 |
| M64269 | PB-derived | 9713 | 0.1190 | |
| M73720 | Carboxypeptidase A | CB-derived | 56 677 | 0.8558 |
| M73720 | PB-derived | 63 256 | 0.7748 | |
| X06182 | c-kit | CB-derived | 11 124 | 0.1680 |
| X06182 | PB-derived | 10 513 | 0.1288 | |
| M33195 | FcR γ chain | CB-derived | 14 214 | 0.2146 |
| M33195 | PB-derived | 16 962 | 0.2078 | |
| M10277 | β-actin | CB-derived | 66 230 | 1 |
| M10277 | PB-derived | 81 637 | 1 |
| Accession no. . | Gene name . | MCs . | 4-150Average difference . | Ratio to β-actin . |
|---|---|---|---|---|
| X67325 | CDK inhibitor, p27kip1 | CB-derived | −1442 | −0.0218 |
| X67325 | PB-derived | 9683 | 0.1186 | |
| X06948 | FcεRI α chain | CB-derived | 761 | 0.0115 |
| X06948 | PB-derived | 8215 | 0.1006 | |
| M34996 | HLA-DQA | CB-derived | 2511 | 0.0379 |
| M34996 | PB-derived | 13 949 | 0.1709 | |
| M89796 | FcεRI β chain | CB-derived | 4057 | 0.0613 |
| M89796 | PB-derived | 15 706 | 0.1924 | |
| M63379 | TRPM-2 | CB-derived | 34 450 | 0.5202 |
| M63379 | PB-derived | 123 684 | 1.5150 | |
| X03068 | HLA-DQw1.1 β chain | CB-derived | 2387 | 0.0360 |
| X03068 | PB-derived | 8243 | 0.1010 | |
| HG4069 | Monocyte chemotactic protein | CB-derived | 7113 | 0.1074 |
| HG4069 | PB-derived | 22 056 | 0.2702 | |
| U50136 | Leukotriene C4 synthase | CB-derived | 9581 | 0.1447 |
| U50136 | PB-derived | 26 175 | 0.3206 | |
| J04990 | Cathepsin G | CB-derived | 27 165 | 0.4102 |
| J04990 | PB-derived | 26 756 | 0.3277 | |
| M33493 | Tryptase | CB-derived | 107 446 | 1.6223 |
| M33493 | PB-derived | 123 746 | 1.5158 | |
| M64269 | Chymase | CB-derived | 5556 | 0.0839 |
| M64269 | PB-derived | 9713 | 0.1190 | |
| M73720 | Carboxypeptidase A | CB-derived | 56 677 | 0.8558 |
| M73720 | PB-derived | 63 256 | 0.7748 | |
| X06182 | c-kit | CB-derived | 11 124 | 0.1680 |
| X06182 | PB-derived | 10 513 | 0.1288 | |
| M33195 | FcR γ chain | CB-derived | 14 214 | 0.2146 |
| M33195 | PB-derived | 16 962 | 0.2078 | |
| M10277 | β-actin | CB-derived | 66 230 | 1 |
| M10277 | PB-derived | 81 637 | 1 |
Among approximately 5600 genes, only 8 genes (shown on the upper 16 lines) were expressed greater than 10% of β-actin mRNA and were significantly increased (more than 2 times) in PB-derived MCs. In the lower lines, 6 mast cell-specific genes are shown. Thirteen pairs of the FcεRI β chain probes indicated that the mRNA is present and the remaining 7 probes indicated that it is absent, suggesting the presence of a splicing variant in the transcript.
Average differences were obtained by subtracting the fluorescence intensities of mismatched microprobes from those of matched microprobes. Analysis using MCs without IL-4 priming gave similar results, except a lower FcεRIα-to-β-actin ratio (0.0718) in unprimed PB-derived MCs.