Mediators released from MCs by IgE-dependent and -independent stimuli
| . | Treatment . | CB-derived . | n . | PB-derived . | n . | P . |
|---|---|---|---|---|---|---|
| Histamine release (%) | IgE | 1.7 ± 0.6 | 4 | 16.1 ± 2.4 | 9 | <.01 |
| IgE + IL-4 | 9.5 ± 3.3 | 4 | 38.9 ± 5.2 | 9 | <.01 | |
| A23187* | 71.1 ± 4.1 | 4 | 80.8 ± 2.9 | 4 | n.s.† | |
| IL-5 (pg/106MCs) | IgE | 1.5 ± 0.4 | 14 | 14.3 ± 7.8 | 6 | n.s.‡ |
| IgE + IL-4 | 30.8 ± 5.4 | 14 | 215.2 ± 85.9 | 6 | <.005 | |
| GM-CSF (ng/106MCs) | IgE | 0.06 ± 0.01 | 15 | 1.94 ± 0.93 | 6 | <.001 |
| IgE + IL-4 | 0.58 ± 0.06 | 15 | 14.00 ± 3.54 | 6 | <.001 |
| . | Treatment . | CB-derived . | n . | PB-derived . | n . | P . |
|---|---|---|---|---|---|---|
| Histamine release (%) | IgE | 1.7 ± 0.6 | 4 | 16.1 ± 2.4 | 9 | <.01 |
| IgE + IL-4 | 9.5 ± 3.3 | 4 | 38.9 ± 5.2 | 9 | <.01 | |
| A23187* | 71.1 ± 4.1 | 4 | 80.8 ± 2.9 | 4 | n.s.† | |
| IL-5 (pg/106MCs) | IgE | 1.5 ± 0.4 | 14 | 14.3 ± 7.8 | 6 | n.s.‡ |
| IgE + IL-4 | 30.8 ± 5.4 | 14 | 215.2 ± 85.9 | 6 | <.005 | |
| GM-CSF (ng/106MCs) | IgE | 0.06 ± 0.01 | 15 | 1.94 ± 0.93 | 6 | <.001 |
| IgE + IL-4 | 0.58 ± 0.06 | 15 | 14.00 ± 3.54 | 6 | <.001 |
CB-derived MCs or PB-derived MCs were pretreated with 1 μg/mL IgE or IgE + 10 ng/mL IL-4 for 48 hours. After they were washed, the cells were challenged with 1.5 μg/mL rabbit anti-human IgE antibody for 30 minutes (histamine release) or 24 hours (IL-5 and GM-CSF). Each value represents an average ± SEM of the net value (specific value–spontaneous value). Experiment number (n) was 4 to 15, as indicated.
Some of the cells were challenged with ionophore A23817 for histamine release.
Not significant.
Judged as not significant because even PB-derived MCs sometimes failed to produce IL-5 at the detectable levels.