Polymerase chain reaction primers
| For LM-PCR and inverse PCR . | For RT-PCR . | ||
|---|---|---|---|
| BW-1 | 5′-GCGGTGACCCGGGAGATCTGAATTC-3′ | TEL940f | 5′-CTCTCTCATCGGGAAGACCTGGC-3′ |
| BW-2 | 5′-GAATTCAGATC-3′ | TEL981f | 5′-GGTCTCTGTCTCCCCGCCTGAAG-3′ |
| E1F (TEL) | 5′-GGATACCCCTGTGTGTACTCCACTCT-3′ | AML1-r41 | 5′-ATTGCCAGCCATCACAGTGAC-3′ |
| E2F (TEL) | 5′-CTGATGTCTTTCTGGACTCCTCGATC-3′ | AML1-r42 | 5′-CAGAGTGCCATCTGGAACAT-3′ |
| E3F (TEL) | 5′-CTGAGGTGGGAGAATCGCTTGAAC-3′ | AML1-r31 | 5′-AGCGGCAACGCCTCGCTCAT-3′ |
| E4F (TEL) | 5′-GGAAGGTGGAGGTTGTAATGAGCC-3′ | AML1-r32 | 5′-TCATCTTGCCTGGGCTCAGCG-3′ |
| E1R (TEL) | 5′-ACTACAGACTGGCAGAGCTGGAAGG-3′ | GAPDHf | 5′-CCATGGAGAAGGTGGGG-3′ |
| E2R (TEL) | 5′-GGATGATTCAGTCCTACCTTCTTCGG-3′ | GAPDHr | 5′-CAAAGTTGTCATGGATGACC-3′ |
| A1R (AML1) | 5′-CCTAGATCCCTGGACATGCCTTTACC-3′ | ||
| A2R (AML1) | 5′-GTCCTCTCTTCCCTTGGCTAGATACC-3′ | ||
| A3R (AML1) | 5′-TGCCTTAGCGATGTCACTCCTGG-3′ | ||
| A4R (AML1) | 5′-GGATGGGGTAAGGTAAGATGTAGGG-3′ | ||
| B1F (BCR) | 5′-GGAAGGAGAGTCTGAAAGTGCAGGGG-3′ | ||
| B2F (BCR) | 5′-AGCATAGGATACTCAGGTGGCTCTGG-3′ | ||
| BG1F (β-globin) | 5′-GCAACCTCAAACAGACACCATGG-3′ | ||
| BG2F (β-globin) | 5′-GGTGAACGTGGATGAAGTTGGTG-3′ | ||
| G1F (GAPDH) | 5′-GTTCGACAGTCAGCCGCATCTTC-3′ | ||
| G2F (GAPDH) | 5′-ATCGCTCAGACACCATGGGGAAG-3′ | ||
| For LM-PCR and inverse PCR . | For RT-PCR . | ||
|---|---|---|---|
| BW-1 | 5′-GCGGTGACCCGGGAGATCTGAATTC-3′ | TEL940f | 5′-CTCTCTCATCGGGAAGACCTGGC-3′ |
| BW-2 | 5′-GAATTCAGATC-3′ | TEL981f | 5′-GGTCTCTGTCTCCCCGCCTGAAG-3′ |
| E1F (TEL) | 5′-GGATACCCCTGTGTGTACTCCACTCT-3′ | AML1-r41 | 5′-ATTGCCAGCCATCACAGTGAC-3′ |
| E2F (TEL) | 5′-CTGATGTCTTTCTGGACTCCTCGATC-3′ | AML1-r42 | 5′-CAGAGTGCCATCTGGAACAT-3′ |
| E3F (TEL) | 5′-CTGAGGTGGGAGAATCGCTTGAAC-3′ | AML1-r31 | 5′-AGCGGCAACGCCTCGCTCAT-3′ |
| E4F (TEL) | 5′-GGAAGGTGGAGGTTGTAATGAGCC-3′ | AML1-r32 | 5′-TCATCTTGCCTGGGCTCAGCG-3′ |
| E1R (TEL) | 5′-ACTACAGACTGGCAGAGCTGGAAGG-3′ | GAPDHf | 5′-CCATGGAGAAGGTGGGG-3′ |
| E2R (TEL) | 5′-GGATGATTCAGTCCTACCTTCTTCGG-3′ | GAPDHr | 5′-CAAAGTTGTCATGGATGACC-3′ |
| A1R (AML1) | 5′-CCTAGATCCCTGGACATGCCTTTACC-3′ | ||
| A2R (AML1) | 5′-GTCCTCTCTTCCCTTGGCTAGATACC-3′ | ||
| A3R (AML1) | 5′-TGCCTTAGCGATGTCACTCCTGG-3′ | ||
| A4R (AML1) | 5′-GGATGGGGTAAGGTAAGATGTAGGG-3′ | ||
| B1F (BCR) | 5′-GGAAGGAGAGTCTGAAAGTGCAGGGG-3′ | ||
| B2F (BCR) | 5′-AGCATAGGATACTCAGGTGGCTCTGG-3′ | ||
| BG1F (β-globin) | 5′-GCAACCTCAAACAGACACCATGG-3′ | ||
| BG2F (β-globin) | 5′-GGTGAACGTGGATGAAGTTGGTG-3′ | ||
| G1F (GAPDH) | 5′-GTTCGACAGTCAGCCGCATCTTC-3′ | ||
| G2F (GAPDH) | 5′-ATCGCTCAGACACCATGGGGAAG-3′ | ||
LM-PCR indicates ligation-mediated polymerase chain reaction; RT-PCR, reverse transcriptase-PCR.