Primers used for PCR amplification of NCF-1
| Primers for gDNA analysis . | Primers for cDNA analysis . |
|---|---|
| 5′L gcctggataacaaagcaaga | cDNA1F ATGGGGGACACCTTCATCCGTCACA |
| 5′L2 gaactgtgatccaccactgcact | cDNA1R AGGGCGATGTGACGGATGAAGGT |
| 5′L3 cggaagaggtgacatttga | cDNA3F GAGGCAGGGGCGATCAATC |
| 5′R1 ggaaaagggtttacacttgt | cDNA4R GTCATCAGGGCGCACCTTGAA |
| il-3′F ggtccacgtttgtgccct | cDNA6F CCCATCATCCTGCAGACGTA |
| 1L agcgacttcctctttcca | cDNA7F GTGGTTCTGTCAGATGAAAGC |
| 1R ctgtccctcccgtattcc | cDNA7R CTCAGGGTCTTCCGTCTCGT |
| 2LA caatcctctgggcttcct | cDNA9F GCCCAACGCCAGATCAAGC |
| 2RA ccctttaactgtggtctgg | cDNA9R CCCCGCTTGATCTGGCGTTG |
| 2LB2 gtgcacacagcaaagcctct | cDNA11R CAGACGCCAGCTTCCGCTTG |
| 2RB2 ctaaggtccttcccaaagggt | |
| 3L ccaatctcgtgcttttccaa | |
| 3R gccaatgaccccctgaca | |
| 4L ccctctcgggcttgacct | |
| 4R3 gcaaaacacagaaagtccca | |
| 5L ccagtgcctcttcttcctc | |
| 5R ggtctcctcagggtgtcc | |
| 6L ggcagcagactcaagatg | |
| 6R cttgtggctgtgggttcc | Allele-specific primers |
| 7L gcccctcagtcacattcc | |
| 7R ctcccacacccctacagc | GTGT ctttcccccagGTGTACAT gDNA |
| 8L gctgggccgacctcacact | GTGT-R TCACCAGGAACATGTACAC gDNA/cDNA |
| 8R ctggctggggctgactca | cDNAGTGT CCCAGCCAGCACTATGTGT cDNA |
| 9LA cagaggcaggtgtccggttg | |
| 9RA tgcctctcctgccaggctga | |
| 9L gccctgaaaccctctcctc | |
| 9R cagagcgaacccgtggtg | |
| 10L ggggtgcccatctgagtcc | |
| 10RA tccctctgattccgccctc | |
| 10R ggtcccgcccgctactct | |
| 11LA agagcgcggagcaggagtt | |
| 11LB2 agcgctgtgggcggggccagtgtg | |
| 11R gctgccctcggcgtccag |
| Primers for gDNA analysis . | Primers for cDNA analysis . |
|---|---|
| 5′L gcctggataacaaagcaaga | cDNA1F ATGGGGGACACCTTCATCCGTCACA |
| 5′L2 gaactgtgatccaccactgcact | cDNA1R AGGGCGATGTGACGGATGAAGGT |
| 5′L3 cggaagaggtgacatttga | cDNA3F GAGGCAGGGGCGATCAATC |
| 5′R1 ggaaaagggtttacacttgt | cDNA4R GTCATCAGGGCGCACCTTGAA |
| il-3′F ggtccacgtttgtgccct | cDNA6F CCCATCATCCTGCAGACGTA |
| 1L agcgacttcctctttcca | cDNA7F GTGGTTCTGTCAGATGAAAGC |
| 1R ctgtccctcccgtattcc | cDNA7R CTCAGGGTCTTCCGTCTCGT |
| 2LA caatcctctgggcttcct | cDNA9F GCCCAACGCCAGATCAAGC |
| 2RA ccctttaactgtggtctgg | cDNA9R CCCCGCTTGATCTGGCGTTG |
| 2LB2 gtgcacacagcaaagcctct | cDNA11R CAGACGCCAGCTTCCGCTTG |
| 2RB2 ctaaggtccttcccaaagggt | |
| 3L ccaatctcgtgcttttccaa | |
| 3R gccaatgaccccctgaca | |
| 4L ccctctcgggcttgacct | |
| 4R3 gcaaaacacagaaagtccca | |
| 5L ccagtgcctcttcttcctc | |
| 5R ggtctcctcagggtgtcc | |
| 6L ggcagcagactcaagatg | |
| 6R cttgtggctgtgggttcc | Allele-specific primers |
| 7L gcccctcagtcacattcc | |
| 7R ctcccacacccctacagc | GTGT ctttcccccagGTGTACAT gDNA |
| 8L gctgggccgacctcacact | GTGT-R TCACCAGGAACATGTACAC gDNA/cDNA |
| 8R ctggctggggctgactca | cDNAGTGT CCCAGCCAGCACTATGTGT cDNA |
| 9LA cagaggcaggtgtccggttg | |
| 9RA tgcctctcctgccaggctga | |
| 9L gccctgaaaccctctcctc | |
| 9R cagagcgaacccgtggtg | |
| 10L ggggtgcccatctgagtcc | |
| 10RA tccctctgattccgccctc | |
| 10R ggtcccgcccgctactct | |
| 11LA agagcgcggagcaggagtt | |
| 11LB2 agcgctgtgggcggggccagtgtg | |
| 11R gctgccctcggcgtccag |