Expression of receptors, MHC class II, and costimulatory molecules on IIA1.6 transfectants
| . | FcαR cotransfected with . | ||
|---|---|---|---|
| WT γ chain . | IIA ITAM γ chain . | Y → F γ chain . | |
| FcαR | 81 ± 11 | 84 ± 10 | 76 ± 16 |
| IAd | 110 ± 11 | 139 ± 3 | 224 ± 19 |
| B7-1 | 14 ± 5 | 18 ± 3 | 5.5 ± 3 |
| B7-2 | 15 ± 2 | 0 | 0 |
| ICAM-1 | 959 ± 127 | 609 ± 187 | 573 ± 47 |
| BCR | 187 ± 11 | 221 ± 45 | 167 ± 54 |
| . | FcαR cotransfected with . | ||
|---|---|---|---|
| WT γ chain . | IIA ITAM γ chain . | Y → F γ chain . | |
| FcαR | 81 ± 11 | 84 ± 10 | 76 ± 16 |
| IAd | 110 ± 11 | 139 ± 3 | 224 ± 19 |
| B7-1 | 14 ± 5 | 18 ± 3 | 5.5 ± 3 |
| B7-2 | 15 ± 2 | 0 | 0 |
| ICAM-1 | 959 ± 127 | 609 ± 187 | 573 ± 47 |
| BCR | 187 ± 11 | 221 ± 45 | 167 ± 54 |
IIA1.6 cells expressing FcαR and either WT γ chain, γ chain with IIA ITAM, or γ chain with Y → F mutation were stained for surface expression of FcαR; class II MHC (IAd); costimulatory molecules B7-1, B7-1, and ICAM-1, and surface IgG (BCR), as described in “Materials and methods.” Results are expressed as the mean fluorescence intensity (MFI) of 10 000 cells minus MFI of the isotype control. The results show the mean and SD of 3 separate experiments.