Comparison of the mean fluorescence intensity for membrane receptors on control versus patient neutrophils
.
Control
.
Patient
.
Control
22.0
22.1
αM
320.1
339.3
β2
406.0
374.5
L-selectin
100.9
84.5
PSGL-1
145.7
257.9
.
Control
.
Patient
.
Control
22.0
22.1
αM
320.1
339.3
β2
406.0
374.5
L-selectin
100.9
84.5
PSGL-1
145.7
257.9
Flow cytometric analysis of control and patient neutrophils comparing the expression of αM/β2, L-selectin, and PSGL-1. Patient and control neutrophils were analyzed by flow cytometry as described in “Materials and methods.” An antibody to P-selectin, S12, was used as the negative control mAb. The mAbs 60.1, 60.3, Dreg 200 and PL-2 were used to detect αM, β2, L-selectin, and PSGL-1, respectively.