Intracellular Ca++ increase after thrombin activation
| . | [Ca++]i response (in nM) . | ||
|---|---|---|---|
| Mean . | SEM . | n . | |
| Thrombin | 335 | 39 | 14 |
| Thrombin + wortmannin | 504 | 88 | 8 |
| Thrombin + Btk PH domain | 384 | 67 | 7 |
| XID + thrombin | 273 | 63 | 4 |
| . | [Ca++]i response (in nM) . | ||
|---|---|---|---|
| Mean . | SEM . | n . | |
| Thrombin | 335 | 39 | 14 |
| Thrombin + wortmannin | 504 | 88 | 8 |
| Thrombin + Btk PH domain | 384 | 67 | 7 |
| XID + thrombin | 273 | 63 | 4 |
Mouse megakaryocytes microinjected with Fura-2 were stimulated by thrombin under control conditions, after treatment for 15 minutes with wortmannin (100 nM), after microinjection of the wild-type PH domain of Btk, and in XID mice. [Ca++]i was measured using single-cell imaging. The increase of [Ca++]i is shown as mean ± SEM for the number of cells indicated. An ANOVA test of the calcium response under these conditions shows no significance.
[Ca++]I indicates intracellular Ca++; Btk, Bruton's tyrosine kinase; PH, pleckstrin homology.