Effects of E2F-1 overexpression on cell cycle S phase in the presence and absence of cytokine growth factor
| . | Cells in S phase at 24 hours (%) . | |
|---|---|---|
| IL-3 . | No cytokine . | |
| Control cells | 58.5 | 7.5 |
| E2F-1–overexpressing cells | 72.1 | 62.6 |
| . | Cells in S phase at 24 hours (%) . | |
|---|---|---|
| IL-3 . | No cytokine . | |
| Control cells | 58.5 | 7.5 |
| E2F-1–overexpressing cells | 72.1 | 62.6 |
Cells were cultured with or without interleukin (IL)–3 for 24 hours and then fixed in 70% ethanol prior to staining with propidium iodide. Cell cycle analysis was performed on the nonapoptotic (viable) cell population by flow cytometry. Shown are the mean results for the percentage of cells in S phase, as determined from 3 separate experiments.