Table 4. NH 2 terminal amino acid... | American Society of Hematology

Table 4.

NH₂ terminal amino acid sequence analysis of the myotube-synthesized factor IX

Cycle	Amino acid	pmol	Cycle	Amino acid	pmol
1	Tyr	76.2	20	(Glu)	—^4-150
2	Asn	66.5	21	(Glu)	—^4-150
3	Ser	34.1	22	Lys	49.7
4	Gly	56.1	23	(Cys)	—^4-150
5	Lys	76.6	24	Ser	12.6
6	Leu	68.8	25	Phe	28.3
7	(Glu)	—^4-150	26	(Glu)	—^4-150
8	(Glu)	—^4-150	27	(Glu)	—^4-150
9	Phe	64.4	28	Ala	33.9
10	Val	72.6	29	Arg	32.3
11	Gln	54.7	30	(Glu)	—^4-150
12	Gly	44.7	31	Val	10.5
13	Asn	39.4	32	Phe	12.8
14	Leu	59.4	33	(Glu)	—^4-150
15	(Glu)	—^4-150	34	Asn	8.9
16	Arg	82.4	35	Thr	11.5
17	(Glu)	—^4-150	36	(Glu)	—^4-150
18	(Cys)	—^4-150	37	Arg	12.1
19	Met	64.0

Cycle	Amino acid	pmol	Cycle	Amino acid	pmol
1	Tyr	76.2	20	(Glu)	—^4-150
2	Asn	66.5	21	(Glu)	—^4-150
3	Ser	34.1	22	Lys	49.7
4	Gly	56.1	23	(Cys)	—^4-150
5	Lys	76.6	24	Ser	12.6
6	Leu	68.8	25	Phe	28.3
7	(Glu)	—^4-150	26	(Glu)	—^4-150
8	(Glu)	—^4-150	27	(Glu)	—^4-150
9	Phe	64.4	28	Ala	33.9
10	Val	72.6	29	Arg	32.3
11	Gln	54.7	30	(Glu)	—^4-150
12	Gly	44.7	31	Val	10.5
13	Asn	39.4	32	Phe	12.8
14	Leu	59.4	33	(Glu)	—^4-150
15	(Glu)	—^4-150	34	Asn	8.9
16	Arg	82.4	35	Thr	11.5
17	(Glu)	—^4-150	36	(Glu)	—^4-150
18	(Cys)	—^4-150	37	Arg	12.1
19	Met	64.0

Picomole amounts of residual amino acids were at or below background levels.