Specific activity of myotube-synthesized F.IX in the conditioned medium from myotubes transduced with AAV-CMV-hF.IX at a range of MOI
| MOI . | [FIX] ELISA (mg/mL) . | Activity (U/mL) . | Specific activity (U/mg) . |
|---|---|---|---|
| 5000 | 0.071 | 0.0135 | 188.5 |
| 10 000 | 0.265 | 0.062 | 233 |
| 20 000 | 0.221 | 0.047 | 212 |
| 40 000 | 0.396 | 0.058 | 146 |
| 80 000 | 0.656 | 0.0712 | 108 |
| mock | 0 | 0.001 | — |
| MOI . | [FIX] ELISA (mg/mL) . | Activity (U/mL) . | Specific activity (U/mg) . |
|---|---|---|---|
| 5000 | 0.071 | 0.0135 | 188.5 |
| 10 000 | 0.265 | 0.062 | 233 |
| 20 000 | 0.221 | 0.047 | 212 |
| 40 000 | 0.396 | 0.058 | 146 |
| 80 000 | 0.656 | 0.0712 | 108 |
| mock | 0 | 0.001 | — |