Lack of effect of short-term exposure to TGF-β1 on the repopulating activity of cultured CD34+ cord blood cells
| Experiment no. . | Suspension culture + 5 GF . | Suspension culture + 5 GF + TGF-β1 . | BM stroma . | BM stroma + 5 GF . | BM stroma + TGF-β1 . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | |
| 1 | 3.0 | 7/8 | 2.1 | 7/8 | 1.5 | 8/8 | 2.1 | 7/8 | 0.9 | 6/6 |
| 2 | 3.3 | 5/10 | 1.6 | 7/10 | 0.8 | 3/9 | 1.8 | 3/10 | 0.9 | 4/7 |
| 3 | 1.5 | 8/8 | 1.2 | 5/8 | 0.8 | 5/7 | 1.2 | 6/6 | 0.8 | 3/5 |
| Total | 20/26 (77%) | 19/26 (73%) | 16/24 (67%) | 16/26 (62%) | 13/18 (72%) | |||||
| Experiment no. . | Suspension culture + 5 GF . | Suspension culture + 5 GF + TGF-β1 . | BM stroma . | BM stroma + 5 GF . | BM stroma + TGF-β1 . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | Cells per mouse3-150 (×106) . | L+ + M+ . | |
| 1 | 3.0 | 7/8 | 2.1 | 7/8 | 1.5 | 8/8 | 2.1 | 7/8 | 0.9 | 6/6 |
| 2 | 3.3 | 5/10 | 1.6 | 7/10 | 0.8 | 3/9 | 1.8 | 3/10 | 0.9 | 4/7 |
| 3 | 1.5 | 8/8 | 1.2 | 5/8 | 0.8 | 5/7 | 1.2 | 6/6 | 0.8 | 3/5 |
| Total | 20/26 (77%) | 19/26 (73%) | 16/24 (67%) | 16/26 (62%) | 13/18 (72%) | |||||
TGF-β1 indicates transforming growth factor β1; BM, bone marrow.
More than 90% of all the cells from each culture were injected into a minimum of 8 irradiated NOD/SCID mice in each experiment. 5GF = FL + SF + IL-3 + IL-6 + G-CSF, as described in “Materials and methods.”