Influence of protease and kinase inhibitors on the release of sγc
| Inhibitor . | sγc (ng/ml) . | sIL4Rα (ng/ml) . |
|---|---|---|
| None | 15.9 | 2.4 |
| TAPI (200 mM) | 15.8 | 1.3 |
| KB8301 (10 μM) | 15.7 | ND |
| EDTA-Na2 (400 μg/ml) | 14.2 | ND |
| ALLN (2 μM) | 15.3 | ND |
| Antipain (33 μg/ml) | 19.8 | ND |
| Rapamycin (200 ng/ml) | 15.8 | 1.4 |
| Staurosporin (5 nM) | 16.7 | 0.7 |
| Inhibitor . | sγc (ng/ml) . | sIL4Rα (ng/ml) . |
|---|---|---|
| None | 15.9 | 2.4 |
| TAPI (200 mM) | 15.8 | 1.3 |
| KB8301 (10 μM) | 15.7 | ND |
| EDTA-Na2 (400 μg/ml) | 14.2 | ND |
| ALLN (2 μM) | 15.3 | ND |
| Antipain (33 μg/ml) | 19.8 | ND |
| Rapamycin (200 ng/ml) | 15.8 | 1.4 |
| Staurosporin (5 nM) | 16.7 | 0.7 |
Spleen cells obtained from BALB/c mice were cultured in the presence of Con A (7.5 μg/ml), and the supernatants were analyzed by ELISA for the sγc or the sIL-4Rα. All inhibitors were added simultaneously with the respective stimulus and used in nontoxic concentrations as determined by trypan blue dye exclusion. The data represent the mean of triplicate determinations of one of three identical experiments. Similar results were obtained when PMA (500 ng/ml) or an αCD3 mAb (5 μg/ml) were used to stimulate the cells (data not shown).