Analysis of long-term expression of human glucose-6-phosphate dehydrogenase after syngeneic bone marrow transplantation with transduced hematopoietic stem cells
| . | Primary BMT . | Secondary BMT . |
|---|---|---|
| Mice transplanted, n | 26* | 26† |
| Mice expressing hG6PD, n | 25 | 22 |
| Mice with long-term expression, n (follow-up, mo) | 16 (4-20) | 14 (6-16) |
| Mice with short-term‡ expression, n | 9 | 8 |
| Provirus1-153/nonexpressing mice1-155 | 0/10 | 0/12 |
| Chimerism1-155%, mean ± SD (range) | 40 ± 14 (22-66) | ND |
| Provirus copy number per donor cell,1-154 mean ± SD (range) | 2.8 ± 2.1 (0.9-7.6) | ND |
| . | Primary BMT . | Secondary BMT . |
|---|---|---|
| Mice transplanted, n | 26* | 26† |
| Mice expressing hG6PD, n | 25 | 22 |
| Mice with long-term expression, n (follow-up, mo) | 16 (4-20) | 14 (6-16) |
| Mice with short-term‡ expression, n | 9 | 8 |
| Provirus1-153/nonexpressing mice1-155 | 0/10 | 0/12 |
| Chimerism1-155%, mean ± SD (range) | 40 ± 14 (22-66) | ND |
| Provirus copy number per donor cell,1-154 mean ± SD (range) | 2.8 ± 2.1 (0.9-7.6) | ND |
BMT indicates bone marrow transplantation; hG6PD, human glucose-6-phosphate dehydrogenase; ND, not determined.
Mice with donor hematopoiesis.
From 8 primary recipients.
Mice that lost hG6PD expression within 3 to 4 months.
Presence of the provirus detected by Southern blotting.
Mice that failed or ceased to express hG6PD.
See “Materials and methods.”