Number of colonies derived from osteoclast precursor cells
| . | c-Fms+RANK− . | c-Fms+RANK+ . | c-Fms+RANK− . |
|---|---|---|---|
| No factor | 0 | 0 | 0 |
| M-CSF alone | 0.5 ± 0.5 | 0.8 ± 1.0 | 50.4 ± 10.4 |
| M-CSF + sRANKL | 0.3 ± 0.5 | 0.3 ± 1.0 | 54.8 ± 10.5 |
| . | c-Fms+RANK− . | c-Fms+RANK+ . | c-Fms+RANK− . |
|---|---|---|---|
| No factor | 0 | 0 | 0 |
| M-CSF alone | 0.5 ± 0.5 | 0.8 ± 1.0 | 50.4 ± 10.4 |
| M-CSF + sRANKL | 0.3 ± 0.5 | 0.3 ± 1.0 | 54.8 ± 10.5 |
We cultured 3000 fractionated bone marrow mononuclear cells in methylcellulose medium with or without M-CSF or M-CSF and sRANKL for 6 days. The number of colonies (mean ± SD) was obtained from 3 separate experiments.