Distribution of stromal cells and stromal cell–associated hematopoietic progenitor cells in the buffy coat and hematon compartments
| Bone marrow fractions* . | TNC per femur . | Fibroblast-CFU† . | Native cobblestone area‡ . | LTC-IC/CAFC (d 35)1-153 . | |||
|---|---|---|---|---|---|---|---|
| 1 per × . | Per femur . | 1 per × . | Per femur . | 1 per × . | Per femur . | ||
| Buffy coat | 30.2 ± 2.0 × 106 | 12.5 ± 3.0 × 103 | 2160 ± 480 | 350 ± 25 × 103 | 77 ± 24 | 22.6 ± 3.0 × 103 | 1194 ± 125 |
| Hematon | 3.2 ± 1.2 × 106 | 1.5 ± 0.3 × 103 | 1630 ± 370 | 5.2 ± 1 × 103 | 616 ± 46 | 6.8 ± 1 × 103 | 470 ± 65 |
| Fold difference | × 0.105 | × 8.3 | × 0.75 | × 67 | × 8 | × 3.6 | × 0.39 |
| Pvalue | < .001 | < .001 | > .05 | < .001 | < .001 | < .01 | < .01 |
| Bone marrow fractions* . | TNC per femur . | Fibroblast-CFU† . | Native cobblestone area‡ . | LTC-IC/CAFC (d 35)1-153 . | |||
|---|---|---|---|---|---|---|---|
| 1 per × . | Per femur . | 1 per × . | Per femur . | 1 per × . | Per femur . | ||
| Buffy coat | 30.2 ± 2.0 × 106 | 12.5 ± 3.0 × 103 | 2160 ± 480 | 350 ± 25 × 103 | 77 ± 24 | 22.6 ± 3.0 × 103 | 1194 ± 125 |
| Hematon | 3.2 ± 1.2 × 106 | 1.5 ± 0.3 × 103 | 1630 ± 370 | 5.2 ± 1 × 103 | 616 ± 46 | 6.8 ± 1 × 103 | 470 ± 65 |
| Fold difference | × 0.105 | × 8.3 | × 0.75 | × 67 | × 8 | × 3.6 | × 0.39 |
| Pvalue | < .001 | < .001 | > .05 | < .001 | < .001 | < .01 | < .01 |
CFU indicates colony-forming unit; LTC-IC, long-term culture-initiating cell; CAFC, cobblestone area–forming cell; TNC, total nucleated cell; LDA, limiting dilution analysis.
Bone marrow fractions were isolated from adult male C57B6 mice by three 1 g sedimentations of the hematon particles and decantations of the buffy coat.
Frequency of F-CFU was determined using LDA.
Physiological cell aggregates containing native cobblestone area were counted on day 7 in hematon LTC.
LTC-IC/CAFC frequency was determined on day 35 by LDA of test cells on irradiated stromal feeder layer.
Data are the means ± SD from at least 5 determinations.