Table 3. Amino acid sequences of the... | American Society of Hematology

Table 3.

Amino acid sequences of the peptide panel spanning the Rhesus D protein⁵⁶

Peptide	Sequence	Residues
1	SSKYPRSVRRCLPLW	2-16
2	CLPLWALTLEAALIL	12-26
3	AALILLFYFFTHYDA	22-36
4	THYDASLEDQKGLVA	32-46
5	KGLVASYQVGQDLTV	42-56
6	QDLTVMAAIGLGFLT	52-66
7	MAAIGLGFLTSSFRR	57-71
8	LGFLTSSFRRHSWSS	62-76
9	SSFRRHSWSSVAFNL	67-81
10	HSWSSVAFNLFMLAL	72-86
11	FMLALGVQWAILLDG	82-96
12	ILLDGFLSQFPSGKV	92-106
13	FLSQFPSGKVVITLF	97-111
14	PSGKVVITLFSIRLA	102-116
15	VITLFSIRLATMSAL	107-121
16	SIRLATMSALSVLIS	112-126
17	TMSALSVLISVDAVL	117-131
18	SVLISVDAVLGKVNL	122-136
19	VDAVLGKVNLAQLVV	127-141
20	GKVNLAQLVVMVLVE	132-146
21	MVLVEVTALGNLRMV	142-156
22	VTALGNLRMVISNIF	147-161
23	NLRMVISNIFNTDYH	152-166
24	ISNIFNTDYHMNMMH	157-171
25	NTDYHMNMMHIYVFA	162-176
26	MNMMHIYVFAAYFGL	167-181
27	IYVFAAYFGLSVAWC	172-186
28	AYFGLSVAWCLPKPL	177-191
29	SVAWCLPKPLPEGTE	182-196
30	LPKPLPEGTEDKDQT	187-201
31	PEGTEDKDQTATIPS	192-206
32	DKDQTATIPSLSAML	197-211
33	ATIPSLSAMLGALFL	202-216
34^3-150	LSAMLGALFLWMFWP	207-221
35^3-150	GALFLWMFWPSFNSA	212-226
36^3-150	WMFWPSFNSALLRSP	217-231
37	SFNSALLRSPIERKN	222-236
38	LLRSPIERKNAVFNT	227-241
39	IERKNAVFNTYYAVA	232-246
40	AVFNTYYAVAVSVVT	237-251
41	YYAVAVSVVTAISGS	242-256
42	AISGSSLAHPQGKIS	252-266
43	SLAHPQGKISKTYVH	257-271
44	QGKISKTYVHSAVLA	262-276
45	KTYVHSAVLAGGVAV	267-281
46	SAVLAGGVAVGTSCH	272-286
47	GTSCHLIPSPWLAMV	282-296
48	WLAMVLGLVAGLISV	292-306
49	LGLVAGLISVGGAKY	297-311
50	GLISVGGAKYLPGCC	302-316
51	GGAKYLPGCCNRVLG	307-321
52	LPGCCNRVLGIPHSS	312-326
53	NRVLGIPHSSIMGYN	317-331
54	IPHSSIMGYNFSLLG	322-336
55	IMGYNFSLLGLLGEI	327-341
56	FSLLGLLGEIIYIVL	332-346
57	LLGEIIYIVLLVLDT	337-351
58	IYIVLLVLDTVGAGN	342-356
59	LVLDTVGAGNGMIGF	347-361
60	VGAGNGMIGFQVLLS	352-366
61	QVLLSIGELSLAIVI	362-376
62	LAIVIALTSGLLTGL	372-386
63	LLTGLLLNLKIWKAP	382-396
64	LLNLKIWKAPHEAKY	387-401
65	IWKAPHEAKYFDDQV	392-406
66	HEAKYFDDQVFWKFP	397-411
67	FDDQVFWKFPHLAVG	402-416
68	DDQVFWKFPHLAVGF	403-417

Peptide	Sequence	Residues
1	SSKYPRSVRRCLPLW	2-16
2	CLPLWALTLEAALIL	12-26
3	AALILLFYFFTHYDA	22-36
4	THYDASLEDQKGLVA	32-46
5	KGLVASYQVGQDLTV	42-56
6	QDLTVMAAIGLGFLT	52-66
7	MAAIGLGFLTSSFRR	57-71
8	LGFLTSSFRRHSWSS	62-76
9	SSFRRHSWSSVAFNL	67-81
10	HSWSSVAFNLFMLAL	72-86
11	FMLALGVQWAILLDG	82-96
12	ILLDGFLSQFPSGKV	92-106
13	FLSQFPSGKVVITLF	97-111
14	PSGKVVITLFSIRLA	102-116
15	VITLFSIRLATMSAL	107-121
16	SIRLATMSALSVLIS	112-126
17	TMSALSVLISVDAVL	117-131
18	SVLISVDAVLGKVNL	122-136
19	VDAVLGKVNLAQLVV	127-141
20	GKVNLAQLVVMVLVE	132-146
21	MVLVEVTALGNLRMV	142-156
22	VTALGNLRMVISNIF	147-161
23	NLRMVISNIFNTDYH	152-166
24	ISNIFNTDYHMNMMH	157-171
25	NTDYHMNMMHIYVFA	162-176
26	MNMMHIYVFAAYFGL	167-181
27	IYVFAAYFGLSVAWC	172-186
28	AYFGLSVAWCLPKPL	177-191
29	SVAWCLPKPLPEGTE	182-196
30	LPKPLPEGTEDKDQT	187-201
31	PEGTEDKDQTATIPS	192-206
32	DKDQTATIPSLSAML	197-211
33	ATIPSLSAMLGALFL	202-216
34^3-150	LSAMLGALFLWMFWP	207-221
35^3-150	GALFLWMFWPSFNSA	212-226
36^3-150	WMFWPSFNSALLRSP	217-231
37	SFNSALLRSPIERKN	222-236
38	LLRSPIERKNAVFNT	227-241
39	IERKNAVFNTYYAVA	232-246
40	AVFNTYYAVAVSVVT	237-251
41	YYAVAVSVVTAISGS	242-256
42	AISGSSLAHPQGKIS	252-266
43	SLAHPQGKISKTYVH	257-271
44	QGKISKTYVHSAVLA	262-276
45	KTYVHSAVLAGGVAV	267-281
46	SAVLAGGVAVGTSCH	272-286
47	GTSCHLIPSPWLAMV	282-296
48	WLAMVLGLVAGLISV	292-306
49	LGLVAGLISVGGAKY	297-311
50	GLISVGGAKYLPGCC	302-316
51	GGAKYLPGCCNRVLG	307-321
52	LPGCCNRVLGIPHSS	312-326
53	NRVLGIPHSSIMGYN	317-331
54	IPHSSIMGYNFSLLG	322-336
55	IMGYNFSLLGLLGEI	327-341
56	FSLLGLLGEIIYIVL	332-346
57	LLGEIIYIVLLVLDT	337-351
58	IYIVLLVLDTVGAGN	342-356
59	LVLDTVGAGNGMIGF	347-361
60	VGAGNGMIGFQVLLS	352-366
61	QVLLSIGELSLAIVI	362-376
62	LAIVIALTSGLLTGL	372-386
63	LLTGLLLNLKIWKAP	382-396
64	LLNLKIWKAPHEAKY	387-401
65	IWKAPHEAKYFDDQV	392-406
66	HEAKYFDDQVFWKFP	397-411
67	FDDQVFWKFPHLAVG	402-416
68	DDQVFWKFPHLAVGF	403-417

Rhesus D–specific polymorphisms are in boldface.

F3-150

Two published sequences5,6 differ only at position 218. The peptides originally synthesized used the widely cited21 variant Ile218,5 which is now thought to have resulted from a sequencing error.22 Therefore, only data obtained with the variant Met218,6 as shown here, are included in this report.

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