Rhesus D peptides that stimulate peripheral blood mononuclear cells from naturally alloimmunized RhD-negative women to proliferate in vitro
| Donor . | HLA-DR type . | Serum anti-D antibody (IU/mL*) + other antibody . | No. of pregnancies/most recent pregnancy (y) with RhD+ infant . | RhD peptides stimulating proliferation† . |
|---|---|---|---|---|
| 14 | DRB1*1;4 | 86 + C | 2/1993 | 6 |
| 15 | DRB1*4 | 43 + C | 2/1999 | 2, 3, 6, 7, 9, 10, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 25, 26, 27, 28, 40, 45, 46, 48, 56, 58, 60, 61, 62, 63, 65, 68 |
| 16 | DRB1*3;11 | 6.1 + C | 2/1987 | 2, 3, 4, 5, 6, 7, 8, 11, 13, 16, 17, 18, 19, 21, 22, 23, 24, 28, 29, 32, 38, 40, 46, 47, 52, 53, 55, 56, 58, 59,61, 64 |
| 17 | DRB1*15;3 | 132 + C | 3/1996 | 2, 3,6, 12, 1619, 22, 26, 28, 32, 40, 53, 54, 56, 59, 60, 62 |
| 18 | DRB1*1;15 | 7 | 1/1984 | 17 |
| 19 | DRB1*3;4 | 5 | NK/NK | 6, 7, 9, 10, 13, 14, 16, 17, 21, 22, 28, 46, 53, 55, 60, 61, 62 |
| 20 | NK | 1/1 | 1/1994 | 6 |
| 21 | DRB1*4;13 | 1/2560 | 1/1997 | 9, 13, 16, 63, 68 |
| 22 | DRB1*7;8 | 1/32 | 3/1971 | 2, 17, 28 |
| Donor . | HLA-DR type . | Serum anti-D antibody (IU/mL*) + other antibody . | No. of pregnancies/most recent pregnancy (y) with RhD+ infant . | RhD peptides stimulating proliferation† . |
|---|---|---|---|---|
| 14 | DRB1*1;4 | 86 + C | 2/1993 | 6 |
| 15 | DRB1*4 | 43 + C | 2/1999 | 2, 3, 6, 7, 9, 10, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 25, 26, 27, 28, 40, 45, 46, 48, 56, 58, 60, 61, 62, 63, 65, 68 |
| 16 | DRB1*3;11 | 6.1 + C | 2/1987 | 2, 3, 4, 5, 6, 7, 8, 11, 13, 16, 17, 18, 19, 21, 22, 23, 24, 28, 29, 32, 38, 40, 46, 47, 52, 53, 55, 56, 58, 59,61, 64 |
| 17 | DRB1*15;3 | 132 + C | 3/1996 | 2, 3,6, 12, 1619, 22, 26, 28, 32, 40, 53, 54, 56, 59, 60, 62 |
| 18 | DRB1*1;15 | 7 | 1/1984 | 17 |
| 19 | DRB1*3;4 | 5 | NK/NK | 6, 7, 9, 10, 13, 14, 16, 17, 21, 22, 28, 46, 53, 55, 60, 61, 62 |
| 20 | NK | 1/1 | 1/1994 | 6 |
| 21 | DRB1*4;13 | 1/2560 | 1/1997 | 9, 13, 16, 63, 68 |
| 22 | DRB1*7;8 | 1/32 | 3/1971 | 2, 17, 28 |
Peptides containing Rhesus D–specific polymorphisms are in boldface. The results are combined if more than one sample was tested.
RhD indicates Rhesus D; NK, not known.
Quantified against national reference standards; if tested more than once, highest level is shown. For donors 20, 21, and 22 (whole-blood donors), the value shown is the antibody titer determined by indirect antiglobulin test.
Peptides 34, 35, and 36 containing Met218 were not tested.